Tyutereva Elena V, Dmitrieva Valeria A, Shavarda Alexey L, Voitsekhovskaja Olga V
Laboratory of Molecular and Ecological Physiology, Komarov Botanical Institute, Russian Academy of Sciences, ul. Professora Popova 2, 197376 Saint-Petersburg, Russia.
Laboratory of Analytical Phytochemistry, Komarov Botanical Institute, Russian Academy of Sciences, ul. Professora Popova 2, 197376 Saint-Petersburg, Russia.
Funct Plant Biol. 2018 Mar;45(4):453-463. doi: 10.1071/FP17056.
The barley (Hordeum vulgare L.) chlorina f2 3613 mutant exhibits low photosynthesis and slow growth. This results from downregulation of the levels of photosynthetic antenna proteins caused by the absence of chl b, the major regulator of photosynthetic antennae in land plants. Here, we demonstrate that, when grown in the field in full sunlight, this mutant displays a changed pattern of stomatal responses compared with the parental wild-type cultivar Donaria. However, stomatal regulation of chlorina f2 3613 plants was restored when plants were placed under a shade cover for several days. The shade cover reduced incident PAR from 2000-2200μmolm-2s-1 to 800-880μmolm-2s-1 as measured at noon. Contents of ABA, the xanthophyll precursors of ABA biosynthesis and minor antenna proteins, as well as reactive oxygen species levels in stomata and the sensitivity of stomata to exogenously supplied ABA, were determined in leaves of wild-type Donaria and chlorina f2 3613 before and after shading. The results support the view that the restoration of stomatal control in barley chlorina f2 3613 is correlated with an increase in the levels of the minor antenna protein Lhcb6, which has recently been implicated in the enhancement of stomatal sensitivity to ABA in Arabidopsis thaliana (L.) Heynh.
大麦（Hordeum vulgare L.）的叶绿素f2 3613突变体表现出光合作用低下和生长缓慢的特征。这是由于缺乏叶绿素b导致光合天线蛋白水平下调所致，叶绿素b是陆地植物光合天线的主要调节因子。在此，我们证明，当在全日照的田间生长时，与亲本野生型品种多纳里亚相比，该突变体表现出气孔反应模式的改变。然而，当将叶绿素f2 3613植株置于遮阳罩下数天后，其气孔调节功能得以恢复。遮阳罩使中午测得的入射光合有效辐射从2000 - 2200μmol·m⁻²·s⁻¹降低至800 - 880μmol·m⁻²·s⁻¹。我们测定了野生型多纳里亚和叶绿素f2 3613叶片在遮光前后脱落酸（ABA）的含量、ABA生物合成的叶黄素前体和次要天线蛋白的含量，以及气孔中的活性氧水平和气孔对外源供应ABA的敏感性。结果支持这样一种观点，即大麦叶绿素f2 3613气孔控制功能的恢复与次要天线蛋白Lhcb6水平的增加相关，最近的研究表明该蛋白与拟南芥气孔对ABA敏感性的增强有关。
