Chang C Y, Wu D A, Lai C C, Miller W L, Chung B C
Institute of Molecular Biology, Academia Sinica, Taiwan, Republic of China.
DNA. 1988 Nov;7(9):609-15. doi: 10.1089/dna.1988.7.609.
Adrenodoxin is an iron-sulfur protein that serves as an electron transport intermediate for all mitochondrial forms of cytochrome P450. To facilitate studying the regulation of adrenodoxin, we have cloned and determined the structure of the human adrenodoxin gene. It spans more than 20 kb, containing four exons and three introns. The first exon encodes the 60-amino-acid signal peptide, directing transport of the protein into the inner mitochondrial matrix. The mature peptide of 124 amino acids is encoded by the other three exons. The third exon encodes the portion of the protein containing the iron-sulfur center and a domain which binds other components of the electron transport chain. The transcriptional start sites were determined by primer extension and S1 nuclease mapping. The 5'-flanking region of this gene contains canonical promoters including a TATA box at nucleotide position -30 and two GC boxes at nucleotide positions -60 and -100. The sequence at nucleotides -234 to -252 is also highly homologous to the glucocorticoid-responsive element and the estrogen-responsive element.
肾上腺铁氧还蛋白是一种铁硫蛋白，作为所有线粒体形式的细胞色素P450的电子传递中间体。为便于研究肾上腺铁氧还蛋白的调控，我们克隆并确定了人肾上腺铁氧还蛋白基因的结构。它跨度超过20kb，包含四个外显子和三个内含子。第一个外显子编码60个氨基酸的信号肽，指导蛋白质转运到线粒体内基质中。另外三个外显子编码124个氨基酸的成熟肽。第三个外显子编码蛋白质中包含铁硫中心和与电子传递链其他成分结合的结构域的部分。转录起始位点通过引物延伸和S1核酸酶作图确定。该基因的5'侧翼区域包含典型启动子，包括核苷酸位置-30处的TATA盒和核苷酸位置-60和-100处的两个GC盒。核苷酸-234至-252处的序列也与糖皮质激素反应元件和雌激素反应元件高度同源。
