Patel P D, Sherman T G, Watson S J
Mental Health Research Institute, University of Michigan Medical School, Ann Arbor 48109.
DNA. 1988 Nov;7(9):627-35. doi: 10.1089/dna.1988.7.627.
An observation from high-pressure liquid chromatography (HPLC) suggesting that monkey beta-endorphin (BE) was chemically different from human or rat BE was investigated by determining the cDNA sequence for the monkey pro-opiomelanocortin (POMC) precursor. A full-length cDNA for POMC was isolated from a Macaca nemestrina whole pituitary cDNA library. The longest open reading frame predicts a 264-residue polypeptide exhibiting the basic structure of POMC that is closely homologous to the human counterpart. The monkey BE sequence apparently diverged from the human sequence after the latter had made the His-27 to Tyr-27 change but prior to the Gln-31 to Glu-31 transition, leaving it more hydrophobic than rat or human BE, consistent with its chromatography on reverse-phase HPLC. Comparison of the monkey POMC precursor with those of other species highlights conserved domains, presumably reflecting regions of physiological activity that await elucidation.
通过测定猴阿片促黑皮质素原（POMC）前体的cDNA序列，对高压液相色谱（HPLC）的一项观察结果进行了研究，该观察结果表明猴β-内啡肽（BE）在化学性质上不同于人或大鼠的BE。从食蟹猴全垂体cDNA文库中分离出POMC的全长cDNA。最长的开放阅读框预测有一个264个残基的多肽，其具有POMC的基本结构，与人的对应物密切同源。猴BE序列显然在人序列发生His-27到Tyr-27的变化之后，但在Gln-31到Glu-31的转变之前就与人序列发生了分歧，这使得它比大鼠或人的BE更具疏水性，这与其在反相HPLC上的色谱行为一致。将猴POMC前体与其他物种的进行比较，突出了保守结构域，推测这些结构域反映了有待阐明的生理活性区域。
