Zhao Wenjie, Zhao Haiping, Li Mingxiao, Huang Chengjun
Institute of Microelectronics, Chinese Academy of Sciences, 3 Beitucheng West Road, Beijing, 100029, China.
School of Future Technology, University of Chinese Academy of Sciences, Beijing, China.
J Transl Med. 2020 Apr 15;18(1):168. doi: 10.1186/s12967-020-02335-7.
Neutrophil chemotaxis plays a vital role in human immune system. Compared with traditional cell migration assays, the emergence of microfluidics provides a new research platform of cell chemotaxis study due to the advantages of visualization, precise control of chemical gradient, and small consumption of reagents. A series of microfluidic devices have been fabricated to study the behavior of neutrophils exposed on controlled, stable, and complex profiles of chemical concentration gradients. In addition, microfluidic technology offers a promising way to integrate the other functions, such as cell culture, separation and analysis into a single chip. Therefore, an overview of recent developments in microfluidic-based neutrophil chemotaxis studies is presented. Meanwhile, the strength and drawbacks of these devices are compared.
中性粒细胞趋化性在人体免疫系统中起着至关重要的作用。与传统的细胞迁移分析方法相比，微流控技术的出现为细胞趋化性研究提供了一个新的研究平台，因为它具有可视化、化学梯度精确控制和试剂消耗少等优点。一系列微流控装置已被制造出来，用于研究中性粒细胞在可控、稳定和复杂的化学浓度梯度分布下的行为。此外，微流控技术为将细胞培养、分离和分析等其他功能集成到单个芯片中提供了一条很有前景的途径。因此，本文对基于微流控的中性粒细胞趋化性研究的最新进展进行了综述。同时，比较了这些装置的优缺点。
