Faculty of Civil Engineering, Environmental Engineering Department, Istanbul Technical University, Maslak, Istanbul 34469, Turkey E-mail:
Faculty of Civil Engineering, Applied Biopolymer and Bioplastics Production Technologies Research Center, Istanbul Technical University, Istanbul, Turkey.
Water Sci Technol. 2020 Jan;81(1):21-28. doi: 10.2166/wst.2020.060.
Food industry wastewater (FIWW) streams with high organic content are among the most suitable and inexpensive candidates for polyhydroxyalkanoate (PHA) biopolymer production. Due to its high organic acid content, pickle industry wastewater (PIWW), can be considered as one of the prospective alternatives to petroleum-based polymers for PHA production. In this context, this study aimed to investigate the production of PHA with enriched microbial culture using PIWW. Two laboratory scale sequencing batch reactors (SBRs) were operated under aerobic dynamic feeding conditions at a sludge retention time of 8 days, with a total cycle duration of 24 hours. SBRs were fed with peptone mixture and PIWW. In-cycle analysis and batch respirometric tests were performed to evaluate PHA storage together with biodegradation kinetics. In-cycle analysis showed that maximum PHA content was 1,820 mgCOD/L, corresponding to 44% in the biomass (ratio of chemical oxygen demand (COD) to volatile suspended solids) for PIWW. Experimental results were also confirmed with activated sludge model simulations. As for the PHA composition, hydroxybutyrate was the major fraction. Model simulations proposed a unique conversion-degradation-storage pathway for the organic acid mixture. This paper presents a novel insight for better understanding of PHA biopolymer production using high saline FIWW.
富含高有机物的食品工业废水（FIWW）是生产聚羟基烷酸酯（PHA）生物聚合物最适宜和最便宜的候选物之一。由于其高有机酸含量，泡菜工业废水（PIWW）可以被认为是生产 PHA 的石油基聚合物的有前途的替代品之一。在这种情况下，本研究旨在利用 PIWW 用富集微生物培养物生产 PHA。两个实验室规模的序批式反应器（SBR）在好氧动态进料条件下运行，污泥停留时间为 8 天，总周期持续时间为 24 小时。SBR 以蛋白胨混合物和 PIWW 为食。进行了在线分析和批式呼吸测量试验，以评估 PHA 储存以及生物降解动力学。在线分析表明，PIWW 的最大 PHA 含量为 1,820 mgCOD/L，对应于生物量中 44%（化学需氧量 (COD) 与挥发性悬浮固体的比值）。实验结果也得到了活性污泥模型模拟的证实。就 PHA 组成而言，羟基丁酸酯是主要成分。模型模拟提出了一种独特的有机酸酸混合物的转化-降解-储存途径。本文为更好地理解使用高盐 FIWW 生产 PHA 生物聚合物提供了新的见解。
