家蚕核型多角体病毒 Bm96 抑制家蚕幼虫中的病毒毒力。
Bombyx mori nucleopolyhedrovirus Bm96 suppresses viral virulence in Bombyx mori larvae.
机构信息
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
出版信息
J Invertebr Pathol. 2020 Jun;173:107374. doi: 10.1016/j.jip.2020.107374. Epub 2020 Apr 12.
Bombyx mori nucleopolyhedrovirus (BmNPV) is a severe pathogen for the domestic silkworm, Bombyx mori. BmNPV harbors over 140 protein-coding genes in its 128.4 kilobase pair-long double-stranded genome. However, many BmNPV genes are still uncharacterized. Here we investigated the role of BmNPV Bm96 in both B. mori cultured cells and larvae. We found that Bm96 is mainly expressed at the late stage of infection and accumulation of Bm96 protein peaks at 24 h post infection (hpi) and declines gradually at 48 hpi in B. mori cultured cells. Compared with the wild-type viruses, Bm96-deletion viruses exhibited higher viral propagation and fast-killing phenotype in B. mori larvae. These results strongly suggest that Bm96 negatively regulates the propagation of BmNPV in B. mori larvae. Furthermore, we observed that larvae infected with Bm96-deletion viruses showed lower locomotory activity at the late stage of infection compared with those infected with the wild-type viruses.
家蚕核型多角体病毒（BmNPV）是家蚕的一种严重病原体。BmNPV 的双链基因组长达 128.4 千碱基对，其中包含超过 140 个蛋白编码基因。然而，许多 BmNPV 基因的功能仍不清楚。在这里，我们研究了 BmNPV Bm96 在 B. mori 培养细胞和幼虫中的作用。我们发现 Bm96 主要在感染后期表达，Bm96 蛋白的积累在感染后 24 小时达到峰值（hpi），并在 48 小时逐渐下降。与野生型病毒相比，Bm96 缺失病毒在 B. mori 幼虫中的病毒繁殖和快速致死表型更高。这些结果强烈表明 Bm96 负调控 BmNPV 在 B. mori 幼虫中的繁殖。此外，我们观察到感染 Bm96 缺失病毒的幼虫在感染后期的运动活性低于感染野生型病毒的幼虫。
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