Chair of Biotechnology, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010, Tartu, Estonia.
Chair of Cell Biology, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010, Tartu, Estonia.
Mol Cell Endocrinol. 2020 Jun 15;510:110816. doi: 10.1016/j.mce.2020.110816. Epub 2020 Apr 12.
Human granulosa cells acquired as leftover from IVF treatment can be used to study infertility problems and are a valuable tool in the research of follicle maturation and ovulation. There is a need for more defined and long-term culture protocols for studying the response of granulosa cells upon treatment with selected hormones/chemicals. In the current study, we tested the effect of adding FGF2, IGF2 and FSH into defined basal medium in order to find culture conditions that would support proliferation of cumulus and mural granulosa cells along with the expression of common granulosa cell type markers such as FSHR, AMHR2, LHR and CYP19A1. We found that FGF2, IGF2 together with FSH helped to retain granulosa cell marker expression while supporting cell survival at least for two weeks of culture. The defined serum-free culture conditions for long-term culturing will be valuable in providing new standards in the research of human granulosa cells.
从体外受精治疗中获得的人卵巢颗粒细胞可用于研究不孕问题，并且是研究卵泡成熟和排卵的有价值的工具。需要更明确和长期的培养方案来研究颗粒细胞对选定激素/化学物质的反应。在本研究中，我们测试了在定义的基础培养基中添加 FGF2、IGF2 和 FSH 的效果，以找到支持卵丘和壁颗粒细胞增殖以及表达常见颗粒细胞类型标志物（如 FSHR、AMHR2、LHR 和 CYP19A1）的培养条件。我们发现 FGF2、IGF2 与 FSH 一起有助于保留颗粒细胞标志物的表达，同时至少在两周的培养中支持细胞存活。这种无血清的定义培养条件将有助于为人类颗粒细胞的研究提供新标准。
