Laboratorio de Virología Molecular, Sede Salto, Centro Universitario Regional, Litoral Norte, Universidad de la República, Gral. Rivera 1350, 50000, Salto, Uruguay.
Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo, 11400, Uruguay.
Virus Res. 2020 Jul 2;283:197976. doi: 10.1016/j.virusres.2020.197976. Epub 2020 Apr 12.
An outbreak of atypical pneumonia caused by a novel Betacoronavirus (βCoV), named SARS-CoV-2 has been declared a public health emergency of international concern by the World Health Organization. In order to gain insight into the emergence, evolution and adaptation of SARS-CoV-2 viruses, a comprehensive analysis of genome composition and codon usage of βCoV circulating in China was performed. A biased nucleotide composition was found for SARS-CoV-2 genome. This bias in genomic composition is reflected in its codon and amino acid usage patterns. The overall codon usage in SARS-CoV-2 is similar among themselves and slightly biased. Most of the highly frequent codons are A- and U-ending, which strongly suggests that mutational bias is the main force shaping codon usage in this virus. Significant differences in relative synonymous codon usage frequencies among SARS-CoV-2 and human cells were found. These differences are due to codon usage preferences.
一种新型的β冠状病毒（βCoV）引起的非典型性肺炎已被世界卫生组织宣布为国际关注的公共卫生紧急事件。为了深入了解 SARS-CoV-2 病毒的出现、进化和适应，对在中国流行的βCoV 的基因组组成和密码子使用进行了全面分析。发现 SARS-CoV-2 基因组存在偏向性核苷酸组成。这种基因组组成的偏差反映在其密码子和氨基酸使用模式上。SARS-CoV-2 的整体密码子使用在彼此之间相似，略有偏向。大多数高频密码子以 A 和 U 结尾，这强烈表明突变偏向是塑造该病毒密码子使用的主要力量。发现 SARS-CoV-2 与人类细胞之间相对同义密码子使用频率存在显著差异。这些差异是由于密码子使用偏好造成的。
