Sandberg G, Stenvinkel C, Kölare S
Department of Histology, Karolinska Institute, Stockholm, Sweden.
Exp Cell Biol. 1988;56(3):159-68.
Guinea pig thymocytes were incubated with normal rabbit serum, which resulted in the death of a great majority of the cells. The remaining rabbit serum-resistant cells, representing less than 10% of the thymocytes, contained euchromatic DNA and were of intermediate size and low density. Functional tests indicated that they were enriched in immunologically mature cells, which responded to the mitogenic lectins phytohemagglutinin and concanavalin A, and were depleted of immature, spontaneously proliferating cells and in cells responding to the thymocyte growth peptide. The described procedure for enrichment of immunologically mature thymus cells in guinea pigs may become useful since glucocorticoid treatment, used in mice for enrichment of mature thymocytes, cannot be used for this purpose in guinea pigs.
将豚鼠胸腺细胞与正常兔血清一起孵育，结果导致绝大多数细胞死亡。其余对兔血清有抗性的细胞，占胸腺细胞的比例不到10%，含有常染色质DNA，大小中等，密度较低。功能测试表明，它们富含免疫成熟细胞，这些细胞对促有丝分裂凝集素植物血凝素和刀豆球蛋白A有反应，并且缺乏未成熟的、自发增殖的细胞以及对胸腺细胞生长肽有反应的细胞。由于在小鼠中用于富集成熟胸腺细胞的糖皮质激素处理不能用于豚鼠，因此所描述的富集豚鼠免疫成熟胸腺细胞的方法可能会有用。
