Maria Munawar, Miao Wentao, Castillo Pablo, Zheng Jingwu
Laboratory of Plant Nematology, Institute of Biotechnology, College of Agriculture & Biotechnology, Zhejiang University , Hangzhou 310058, Zhejiang, P.R. China.
Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario, ceiA3, Avenida Menéndez Pidal s/n, 14004 Córdoba, Spain.
J Nematol. 2020;52:1-13. doi: 10.21307/jofnem-2020-042.
The tea plant is native to China; the country has the greatest tea production areas in the world. In an attempt to investigate the nematode biodiversity associated with the tea plantations of Hangzhou, Zhejiang Province, a population of stunt nematode was detected. This group of nematodes is comprised of migratory ecto-parasites of roots and can subsist on a variety of host plants. Therefore, the detected population was studied carefully using the integrative taxonomy approach and identified as a new species of genus . n. sp. can be characterized by females having six incisures in the lateral field; labial region is dome shaped and slightly offset from the rest of the body having four to five annuli; head framework is weakly developed; deirids are absent; excretory pore is located at the anterior region of basal pharyngeal bulb. Under SEM, the vulva is a transverse slit, vulval lips are elongated and ellipsoidal with epiptygma. The tail is annulated, elongated, and conical having bluntly pointed tip and a terminal hyaline region that forms 21 to 33% of the tail length. Spicule is 22 to 25 μm long, gubernaculum is saucer shaped; bursa is crenated covering the tail until the hyaline tail region. Morphologically, the species is close to , , , , , , and . Phylogenetic relationships of the new species based on D2-D3 expansion domains of 28 S, ITS, and 18 S rRNA genes indicated that n. sp. clustered in a separate clade with . The tea plant is native to China; the country has the greatest tea production areas in the world. In an attempt to investigate the nematode biodiversity associated with the tea plantations of Hangzhou, Zhejiang Province, a population of stunt nematode was detected. This group of nematodes is comprised of migratory ecto-parasites of roots and can subsist on a variety of host plants. Therefore, the detected population was studied carefully using the integrative taxonomy approach and identified as a new species of genus . n. sp. can be characterized by females having six incisures in the lateral field; labial region is dome shaped and slightly offset from the rest of the body having four to five annuli; head framework is weakly developed; deirids are absent; excretory pore is located at the anterior region of basal pharyngeal bulb. Under SEM, the vulva is a transverse slit, vulval lips are elongated and ellipsoidal with epiptygma. The tail is annulated, elongated, and conical having bluntly pointed tip and a terminal hyaline region that forms 21 to 33% of the tail length. Spicule is 22 to 25 μm long, gubernaculum is saucer shaped; bursa is crenated covering the tail until the hyaline tail region. Morphologically, the species is close to , , , , , , and . Phylogenetic relationships of the new species based on D2-D3 expansion domains of 28 S, ITS, and 18 S rRNA genes indicated that n. sp. clustered in a separate clade with .
茶树原产于中国；中国拥有世界上最大的茶叶产区。为了调查浙江省杭州市茶园相关的线虫生物多样性，检测到了一群矮化线虫。这一类线虫由根部迁移性外寄生线虫组成，可寄生于多种寄主植物。因此，利用综合分类学方法对检测到的群体进行了仔细研究，并鉴定为一个新属的新物种。新物种可通过以下特征来描述：雌虫侧区有六条刻线；唇区呈圆顶形，与身体其他部分略有偏移，有四到五个环纹；头部骨架发育较弱；无侧器；排泄孔位于基部咽球的前部区域。在扫描电子显微镜下，阴门是一条横向裂缝，阴门唇拉长呈椭圆形并有阴门盖。尾部有环纹，细长，呈圆锥形，尖端钝圆并有一个末端透明区域，该区域占尾长的21%至33%。交合刺长22至25μm，引带呈碟形；尾翼有锯齿，覆盖尾部直至透明尾区。在形态上该物种与[多个物种名称未给出]接近。基于28S、ITS和18S rRNA基因D2-D3扩展域的新物种系统发育关系表明，新物种与[物种名称未给出]聚集在一个单独的分支中。茶树原产于中国；中国拥有世界上最大的茶叶产区。为了调查浙江省杭州市茶园相关的线虫生物多样性，检测到了一群矮化线虫。这一类线虫由根部迁移性外寄生线虫组成，可寄生于多种寄主植物。因此利用综合分类学方法对检测到的群体进行了仔细研究，并鉴定为一个新属的新物种。新物种可通过以下特征来描述：雌虫侧区有六条刻线；唇区呈圆顶形，与身体其他部分略有偏移,有四到五个环纹；头部骨架发育较弱；无侧器；排泄孔位于基部咽球的前部区域。在扫描电子显微镜下，阴门是一条横向裂缝，阴门唇拉长呈椭圆形并有阴门盖。尾部有环纹，细长，呈圆锥形，尖端钝圆并有一个末端透明区域，该区域占尾长的21%至33%。交合刺长22至25μm，引带呈碟形；尾翼有锯齿，覆盖尾部直至透明尾区。在形态上该物种与[多个物种名称未给出]接近。基于28S、ITS和18S rRNA基因D2-D3扩展域的新物种系统发育关系表明，新物种与[物种名称未给出]聚集在一个单独的分支中。
