Bayer Robin, Artmann Aysegül Temiz, Digel Ilya, Falkenstein Julia, Artmann Gerhard, Creutz Till, Hescheler Jürgen
Institute for Bioengineering, University of Applied Science Aachen, Jülich, Germany,
Institute for Neurophysiology, University Cologne, Cologne, Germany.
Cell Physiol Biochem. 2020 Apr 17;54(3):371-383. doi: 10.33594/000000225.
BACKGROUND/AIMS: This study aimed to establish a precise and well-defined working model, assessing pharmaceutical effects on vascular smooth muscle cell monolayer in-vitro. It describes various analysis techniques to determine the most suitable to measure the biomechanical impact of vasoactive agents by using CellDrum technology.
The so-called CellDrum technology was applied to analyse the biomechanical properties of confluent human aorta muscle cells (haSMC) in monolayer. The cell generated tensions deviations in the range of a few N/m² are evaluated by the CellDrum technology. This study focuses on the dilative and contractive effects of L-type Ca channel agonists and antagonists, respectively. We analyzed the effects of Bay K8644, nifedipine and verapamil. Three different measurement modes were developed and applied to determine the most appropriate analysis technique for the study purpose. These three operation modes are called, particular time mode" (PTM), "long term mode" (LTM) and "real-time mode" (RTM).
It was possible to quantify the biomechanical response of haSMCs to the addition of vasoactive agents using CellDrum technology. Due to the supplementation of 100nM Bay K8644, the tension increased approximately 10.6% from initial tension maximum, whereas, the treatment with nifedipine and verapamil caused a significant decrease in cellular tension: 10nM nifedipine decreased the biomechanical stress around 6,5% and 50nM verapamil by 2,8%, compared to the initial tension maximum. Additionally, all tested measurement modes provide similar results while focusing on different analysis parameters.
The CellDrum technology allows highly sensitive biomechanical stress measurements of cultured haSMC monolayers. The mechanical stress responses evoked by the application of vasoactive calcium channel modulators were quantified functionally (N/m²). All tested operation modes resulted in equal findings, whereas each mode features operation-related data analysis.
背景/目的：本研究旨在建立一个精确且定义明确的工作模型，以评估药物对体外血管平滑肌细胞单层的作用。它描述了各种分析技术，以确定使用CellDrum技术测量血管活性药物生物力学影响的最合适方法。
应用所谓的CellDrum技术分析汇合的人主动脉肌细胞（haSMC）单层的生物力学特性。CellDrum技术可评估细胞产生的张力偏差，偏差范围在几N/m²。本研究分别关注L型钙通道激动剂和拮抗剂的舒张和收缩作用。我们分析了Bay K8644、硝苯地平和维拉帕米的作用。开发并应用了三种不同的测量模式，以确定适合该研究目的的分析技术。这三种操作模式分别称为“特定时间模式”（PTM）、“长期模式”（LTM）和“实时模式”（RTM）。
使用CellDrum技术可以量化haSMC对添加血管活性药物的生物力学反应。由于添加了100nM Bay K8644，张力从初始张力最大值增加了约10.6%，而硝苯地平和维拉帕米处理导致细胞张力显著降低：与初始张力最大值相比，10nM硝苯地平使生物力学应力降低约6.5%，50nM维拉帕米使生物力学应力降低2.8%。此外，所有测试的测量模式在关注不同分析参数时提供了相似的结果。
CellDrum技术可对培养的haSMC单层进行高度敏感的生物力学应力测量。应用血管活性钙通道调节剂引起的机械应力反应在功能上被量化（N/m²）。所有测试的操作模式都得出了相同的结果，而每种模式都有与操作相关的数据分析。
