B.I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, 68 Nezavisimost' Ave, Minsk, 220072, Belarus.
Institute of Physical Organic Chemistry, National Academy of Sciences of Belarus, 13 Surganova str, 220072, Minsk, Belarus.
J Fluoresc. 2020 May;30(3):629-635. doi: 10.1007/s10895-020-02535-w. Epub 2020 Apr 16.
Bright fluorescent probes with enhanced intensities in the fluorescein channel are of great value for plenty of biological applications. To design effective probes one should introduce as many as possible fluorophores to the biomolecule while leaving its native structure as intact as possible. To reach this compromise, we designed and synthesized fluorescein bifluorophores on the 3,5-diaminobenzoic acid scaffold, which allows for insertion of two fluorophores at one modification site of a biomolecule. Rigid structure of the branching linker group allows to minimize self-quenching the fluorophores. However, despite the structure similarities of fluorescein isomers (5-FAM and 6-FAM), different photophysical behavior was observed for the corresponding bifluorophores. Here we made efforts to get insight into these effects with the focus on the media viscosity impact.
具有增强的荧光素通道强度的明亮荧光探针在许多生物应用中非常有价值。为了设计有效的探针，在保持生物分子天然结构尽可能完整的情况下，应将尽可能多的荧光团引入到生物分子中。为了达到这个折衷，我们在 3,5-二氨基苯甲酸支架上设计并合成了荧光素双荧光团，这允许在生物分子的一个修饰位点插入两个荧光团。分支连接基团的刚性结构允许最小化荧光团的自猝灭。然而，尽管荧光素异构体（5-FAM 和 6-FAM）具有结构相似性，但相应的双荧光团表现出不同的光物理行为。在这里，我们努力深入了解这些影响，重点关注介质粘度的影响。
