Chen T M, Pacifico J R, Daly R E
Parke-Davis Pharmaceutical Research Division, Warner-Lambert Company, Morris Plains, New Jersey 07981.
J Chromatogr Sci. 1988 Dec;26(12):636-9. doi: 10.1093/chromsci/26.12.636.
An ion-pair reversed-phase high-pressure liquid chromatographic assay is developed that allows simultaneous quantitation of guaifenesin, dextromethorphan hydrobromide, and sodium benzoate in an expectorant syrup. The method is rapid and accurate. Average recoveries of 99.6, 99.8, and 99.7% with relative standard deviations of 0.5, 0.9, and 0.2% are obtained for guaifenesin, dextromethorphan hydrobromide, and sodium benzoate, respectively, from laboratory prepared samples. Chromatographic conditions are selected to afford a pH that provides adequate separation of guaifenesin, dextromethorphan hydrobromide, sodium benzoate, and sodium saccharin and a detection wavelength that effectively compensates for the great disparity in quantity between guaifenesin and dextromethorphan hydrobromide present in syrups. The relationships between the retention volume of dextromethorphan hydrobromide and the alkyl chain length as well as the concentration of the counterion are studied. The retention profiles for sodium saccharin, guaifenesin, sodium benzoate, and dextromethorphan hydrobromide in the apparent pH range of 2.5 to 6.6 are established.
建立了一种离子对反相高效液相色谱法，可同时定量测定一种祛痰糖浆中的愈创甘油醚、氢溴酸右美沙芬和苯甲酸钠。该方法快速且准确。从实验室制备的样品中分别获得愈创甘油醚、氢溴酸右美沙芬和苯甲酸钠的平均回收率为99.6%、99.8%和99.7%，相对标准偏差分别为0.5%、0.9%和0.2%。选择色谱条件以提供一个pH值，该pH值能使愈创甘油醚、氢溴酸右美沙芬、苯甲酸钠和糖精钠得到充分分离，并选择一个检测波长，该波长能有效补偿糖浆中愈创甘油醚和氢溴酸右美沙芬在含量上的巨大差异。研究了氢溴酸右美沙芬的保留体积与烷基链长度以及抗衡离子浓度之间的关系。建立了糖精钠、愈创甘油醚、苯甲酸钠和氢溴酸右美沙芬在2.5至6.6表观pH范围内的保留图谱。
