Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
University of Wroclaw, Poland.
FEBS J. 2021 Jan;288(1):281-292. doi: 10.1111/febs.15336. Epub 2020 May 10.
Proteogenic dipeptides are intermediates of proteolysis as well as an emerging class of small-molecule regulators with diverse and often dipeptide-specific functions. Herein, prompted by differential accumulation of dipeptides in a high-density Arabidopsis thaliana time-course stress experiment, we decided to pursue an identity of the proteolytic pathway responsible for the buildup of dipeptides under heat conditions. By querying dipeptide accumulation versus available transcript data, autophagy emerged as a top hit. To examine whether autophagy indeed contributes to the accumulation of dipeptides measured in response to heat stress, we characterized the loss-of-function mutants of crucial autophagy proteins to test whether interfering with autophagy would affect dipeptide accumulation in response to the heat treatment. This was indeed the case. This work implicates the involvement of autophagy in the accumulation of proteogenic dipeptides in response to heat stress in Arabidopsis.
蛋白源二肽既是蛋白水解的中间产物，也是一类新兴的小分子调节剂，具有多样化且通常为二肽特异性的功能。在此，受到高密度拟南芥时间进程胁迫实验中二肽积累差异的启发，我们决定探索在热条件下导致二肽积累的蛋白水解途径的身份。通过查询二肽积累与可用转录数据的关系，自噬作为一个重要的发现出现了。为了研究自噬是否确实有助于应对热应激时测量的二肽的积累，我们对关键自噬蛋白的功能丧失突变体进行了特征描述，以测试干扰自噬是否会影响对热处理的二肽积累。事实确实如此。这项工作表明自噬参与了拟南芥对热应激的蛋白源二肽的积累。
