Wang Jing, Zhang Yong, Zhou Meiqi, Zheng Meizhu, Cui Jing, Liu Zhiqiang, Liu Chunming, Liu Shu
The Central Laboratory, Changchun Normal University, Changchun, China.
National Center of Mass Spectrometry in Changchun and Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry and Chemical Biology Laboratory, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Biomed Chromatogr. 2020 Aug;34(8):e4852. doi: 10.1002/bmc.4852. Epub 2020 Jun 8.
Superoxide anion radical scavenger and xanthine oxidase inhibitor play an important role in the treatment of several relevant human diseases. In the present study, ultrafiltration liquid chromatography-mass spectrometry coupled to microplate reader was applied to screen and identify superoxide anion radical scavengers and xanthine oxidase inhibitors from total flavonoids of Ginkgo biloba leaves. As a result, four compounds (quercetin, apigenin, kaempferol and isorhamnetin) were screened as xanthine oxidase inhibitors by ultrafiltration LC-MS, and the 50% scavenging concentration values of the screened flavonoids were lower than those for allopurinol. Lineweaver-Burk plot results indicated that kaempferol was a competitive xanthine oxidase inhibitor; the other flavonoids were all anticompetitive inhibitors. Four flavonoids-rutin, quercetin, kaempferol and isorhamnetin-were screened as superoxide anion radical scavengers by LC-MS. The results demonstrate that the method for screening and evaluation of superoxide anion radical scavenger and xanthine oxidase inhibitor from a complex mixture system is feasible and efficient.
超氧阴离子自由基清除剂和黄嘌呤氧化酶抑制剂在多种相关人类疾病的治疗中发挥着重要作用。在本研究中，采用超滤液相色谱 - 质谱联用微孔板读数器，从银杏叶总黄酮中筛选和鉴定超氧阴离子自由基清除剂和黄嘌呤氧化酶抑制剂。结果，通过超滤液相色谱 - 质谱筛选出四种化合物（槲皮素、芹菜素、山奈酚和异鼠李素）作为黄嘌呤氧化酶抑制剂，筛选出的黄酮类化合物的50%清除浓度值低于别嘌呤醇。Lineweaver - Burk图结果表明，山奈酚是一种竞争性黄嘌呤氧化酶抑制剂；其他黄酮类化合物均为反竞争性抑制剂。通过液相色谱 - 质谱筛选出四种黄酮类化合物（芦丁、槲皮素、山奈酚和异鼠李素）作为超氧阴离子自由基清除剂。结果表明，从复杂混合体系中筛选和评价超氧阴离子自由基清除剂和黄嘌呤氧化酶抑制剂的方法是可行且高效的。
