Fukuoka M, Tanaka A, Yamaha T, Naito K, Takada K, Kobayashi K, Tobe M
Division of Biological Chemistry, National Institute of Hygienic Sciences, Tokyo, Japan.
J Appl Toxicol. 1988 Dec;8(6):411-6. doi: 10.1002/jat.2550080605.
Rats receiving a single oral dose of 286.8 mumol/kg tris(2,3-dibromopropyl)phosphate (Tris-BP) showed pyknosis of the renal tubular epithelial cells on Day 1, necrosis on Day 2, regeneration from Day 3 and large nuclei formation from Day 4. 13C-NMR spectra were applied to clarify changes of the renal low-molecular-weight components in the kidney injured by Tris-BP, and sialic acid and inositol were found as the desired marker components. The lesions produced by Tris-BP were characterized by changes in the renal components and enzyme activities. Increases in sialic acid content of the kidney were observed both on Day 1, suggesting destruction of the epithelial cell membrane, and on Day 5, suggesting regeneration accompanied by an increase of inositol content on Days 7 and 10. Renal activity of cytoplasmic enzyme, alanine aminopeptidase, was decreased on Days 1 and 5 and elevated on Days 7 and 10. Lactate dehydrogenase activity showed a tendency to decrease in the kidneys on Day 1 and to increase significantly on Days 2, 5, 6 and 7.
接受单次口服剂量为286.8微摩尔/千克的三（2,3 - 二溴丙基）磷酸酯（Tris - BP）的大鼠，在第1天显示肾小管上皮细胞固缩，第2天出现坏死，第3天开始再生，第4天出现大核形成。应用13C - NMR光谱来阐明受Tris - BP损伤的肾脏中低分子量成分的变化，发现唾液酸和肌醇是所需的标记成分。Tris - BP产生的病变以肾脏成分和酶活性的变化为特征。在第1天观察到肾脏唾液酸含量增加，提示上皮细胞膜破坏；在第5天也观察到增加，提示再生，同时在第7天和第10天肌醇含量增加。细胞质酶丙氨酸氨基肽酶的肾脏活性在第1天和第5天降低，在第7天和第10天升高。乳酸脱氢酶活性在第1天肾脏中有降低趋势，在第2天、第5天、第6天和第7天显著增加。
