College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, China.
College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, China.
Ecotoxicol Environ Saf. 2020 Jul 1;197:110554. doi: 10.1016/j.ecoenv.2020.110554. Epub 2020 Apr 14.
Copper (Cu) is a toxic substance of heavy metals, and arsenic (As) is a toxic substance of metalloids. They all cause oxidative stress and have been widely studied in recent years. Studies have reported that Cu and As can cause inflammation in chicken brain tissue. To assess the toxicological effects of Cu and/or As chronic exposure on chicken thalamus, we used toxicologically relevant concentrations of Cu and As in the chicken diet for 12 weeks. By comparative analysis, we found that higher malondialdehyde (MDA), total antioxidant capacity (T-AOC), and proinflammatory mediator (NF-κB) were observed in the Cu and/or As co-exposed group, indicating that oxidation stress and inflammation are produced. In addition, we also observed mitochondrial kinetics and the generation of apoptosis. These include the gene and protein expression levels of Drp1, Opa1, Mfn1, Mfn2 and Bcl-2, Bax, p53. In conclusion, we believe that in the chronic poisoning of Cu and/or As, inflammation occurs in the chicken thalamus, causing oxidative stress and mitochondrial kinetics, which eventually leads to apoptosis.
铜（Cu）是重金属的有毒物质，砷（As）是类金属的有毒物质。它们都能引起氧化应激，近年来受到广泛研究。研究报告称，Cu 和 As 会导致鸡脑组织炎症。为了评估 Cu 和/或 As 慢性暴露对鸡丘脑的毒理学影响，我们在鸡饲料中使用了毒理学相关浓度的 Cu 和 As 进行了 12 周的实验。通过对比分析，我们发现 Cu 和/或 As 共暴露组的丙二醛（MDA）、总抗氧化能力（T-AOC）和促炎介质（NF-κB）更高，表明氧化应激和炎症产生。此外，我们还观察到线粒体动力学和细胞凋亡的发生。这包括 Drp1、Opa1、Mfn1、Mfn2 和 Bcl-2、Bax、p53 的基因和蛋白表达水平。总之，我们认为在 Cu 和/或 As 的慢性中毒中，鸡丘脑发生炎症，导致氧化应激和线粒体动力学，最终导致细胞凋亡。
