Laboratory of Analysis, Treatment and Valorization of Pollutants of the envirOnment and Products. Faculty of Pharmacy, University of Monastir, Tunisia.
Laboratory of Analysis, Treatment and Valorization of Pollutants of the envirOnment and Products. Faculty of Pharmacy, University of Monastir, Tunisia.
Res Vet Sci. 2020 Aug;131:51-58. doi: 10.1016/j.rvsc.2020.04.008. Epub 2020 Apr 6.
The use of biological immunostimulants is considered a valuable practice to improve culture conditions in aquaculture sector that may help to increase production and maintain healthy environment. We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae. In order to find out if the EPSs have an effect on the biochemical composition during the trial period, the nutritional value has been evaluated. The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-β) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated. In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrβ, as well as catalase gene in liver. During the period of study, EPSs administration did not affect the fatty acid profiles of larvae, which is balanced. This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass. The present findings provided evidence that the tested EPSs with antibacterial and antioxidant activities can enhance immune response without negative effect on the biochemical composition. The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
使用生物免疫刺激剂被认为是改善水产养殖环境的有价值的方法，这可能有助于提高产量并维持健康的环境。我们进行这项研究是为了评估两种来源于潜在益生菌菌株的胞外多糖（EPS）“EPLB”和“EPB”对欧洲鲈鱼（Dicentrarchus labrax L.）幼鱼免疫和抗氧化状态的潜在影响。为了确定 EPS 在试验期间是否对生化组成有影响，我们评估了其营养价值。结果表明，肠道和头肾中与免疫相关的基因（infg、Il1b、Il8、Il6 和 tcr-β）以及肝脏中清除酶（cat、sod、gr）基因的表达水平发生了变化。事实上，用测试的 EPS 进行饮食补充，显著增强了头肾中与免疫相关的基因的表达，特别是 infg 和 tcrβ，以及肝脏中过氧化氢酶基因的表达。在研究期间，EPS 给药不会影响幼虫的脂肪酸谱，其脂肪酸谱是平衡的。这一点得到了二十二碳六烯酸/二十碳五烯酸比值的证实，并表明 EPLB 和 EPB 可以在不影响欧洲鲈鱼生化组成的情况下进行给药。本研究结果表明，具有抗菌和抗氧化活性的测试 EPS 可以增强免疫反应，而不会对生化组成产生负面影响。所使用的 EPS 可以被认为是欧洲鲈鱼天然功能性水产饲料成分的良好来源。
