Sanguino-Ortiz Wilmer, Espinosa-Ruiz Cristóbal, Esteban Abad María Ángeles, Román Críspulo Perea, Hoyos-Concha José Luis
Department of Hydrobiological Resources, Faculty of Livestock Sciences, Torobajo University Citadel, University of Nariño, 52001, Nariño, Colombia.
Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain.
Fish Physiol Biochem. 2025 Feb 27;51(2):56. doi: 10.1007/s10695-024-01444-0.
This study evaluated the effect of replacing fish meal (FM) with concentrated trout viscera protein hydrolysate (TVPH) on the immune response in juvenile red tilapia (Oreocheromis spp). Five isoenergetic and isoproteic experimental diets were prepared by substituting FM with TVPH at different substitution ratios: 0% (control, D1) 25% (D2), 50% (D3), 75% (D4), and 100% (D5). A total of 180 red tilapia were distributed in 15 tanks. Fish from three tanks were fed daily at 2% of the biomass for 25 days with one of the five diets mentioned above. At the end of the trial, the fish were counted, weighed, measured, and skin mucus and serum samples were obtained to study different parameters related to humoral immunity. The results indicate a 100% survival rate in all fish groups and did not show significant differences in terms of growth and feed efficiency. On the other hand, the fish fed diets D2 and D3 had significantly higher serum protein values. Also, fish fed the D2 or D5 diets had higher lysozyme activity and fish fed the D2 diet also had significantly higher total immunoglobulin levels than fish fed the control diet. In mucus, fish fed the D2 or D4 diets showed significantly higher mucus protein levels than control fish. However, anti-protease and bactericidal activity decreased in fish fed the D5 or D4 diets, respectively. These results demonstrate that the D2 and D3 diets positively modulate the immune response of juvenile red tilapia compared to that of fish fed the control diet.
本研究评估了用浓缩鳟鱼内脏蛋白水解物（TVPH）替代鱼粉（FM）对幼年红罗非鱼（Oreocheromis spp）免疫反应的影响。通过以不同替代比例用TVPH替代FM，制备了五种等能量和等蛋白的实验饲料：0%（对照，D1）、25%（D2）、50%（D3）、75%（D4）和100%（D5）。总共180尾红罗非鱼被分配到15个水箱中。来自三个水箱的鱼每天以生物量的2%投喂上述五种饲料之一，持续25天。试验结束时，对鱼进行计数、称重、测量，并采集皮肤黏液和血清样本，以研究与体液免疫相关的不同参数。结果表明，所有鱼组的存活率均为100%，在生长和饲料效率方面未显示出显著差异。另一方面，投喂D2和D3饲料的鱼血清蛋白值显著更高。此外，投喂D2或D5饲料的鱼溶菌酶活性更高，投喂D2饲料的鱼总免疫球蛋白水平也显著高于投喂对照饲料的鱼。在黏液方面，投喂D2或D4饲料的鱼黏液蛋白水平显著高于对照鱼。然而，投喂D5或D4饲料的鱼抗蛋白酶和杀菌活性分别降低。这些结果表明，与投喂对照饲料的鱼相比，D2和D3饲料对幼年红罗非鱼的免疫反应有积极的调节作用。
