Heilongjiang Cold Region Wetland Ecology and Environment Research Key Laboratory, School of Geography and Tourism, Harbin University, Harbin, China.
College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
Environ Res. 2020 Jul;186:109503. doi: 10.1016/j.envres.2020.109503. Epub 2020 Apr 12.
Adding alkaline into an anaerobic waste activated sludge (WAS) fermentation with thermophilic bacteria pretreatment could efficiently improve short-chain fatty acids (SCFAs) accumulation to 3550 ± 120 mg COD/L. The acidification rate in combined test was 21.2%, while that was 15.6% and 10.7% in sole thermophilic bacteria pretreatment and control tests respectively. Four distinct groups of microbes could be identified with noticeable shifts using the combined pretreatments, and tremendous effects were analyzed on organic content especially of the soluble proteins and SCFAs concentrations. Particularly, alkaline addition would significantly change the functional microbial structures, including the decrease of Caloramator with the function of thermophilic proteolytic and the increase of Acidobacteria TM7 and Petrimonas sp. The results above suggested that alkaline addition could decrease the hydrolytic substances consume by thermotolerance bacteria and final improve SCFAs accumulation in fermentation process.
在有嗜热菌预处理的厌氧剩余活性污泥（WAS）发酵中添加碱性物质可以有效地将短链脂肪酸（SCFAs）积累提高到 3550±120mg COD/L。在联合试验中，酸化率为 21.2%，而在单独的嗜热菌预处理和对照试验中，酸化率分别为 15.6%和 10.7%。使用联合预处理可以识别出四个明显不同的微生物群，并且对有机物质（特别是可溶性蛋白质和 SCFAs 浓度）有巨大的影响。特别是，碱性添加会显著改变功能微生物结构，包括具有嗜热蛋白水解功能的 Caloramator 的减少和 Acidobacteria TM7 和 Petrimonas sp. 的增加。上述结果表明，碱性添加可以减少耐热菌消耗的水解物质，最终提高发酵过程中的 SCFAs 积累。
