Department of Environmental Engineering, Zhejiang University, Hangzhou, Zhejiang, 310058, China; Guangdong Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangzhou, Guangdong, 510316, China; MOE Key laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
Department of Environmental Engineering, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
Chemosphere. 2020 Aug;253:126731. doi: 10.1016/j.chemosphere.2020.126731. Epub 2020 Apr 7.
Bioremediation of Cr(VI) contamination using microorganisms is a promising method for reducing its environmental risks. The objective of this study was to clarify Cr(VI) removal by Penicillium oxalicum SL2 in terms of indirect Cr(VI) reduction by metabolites, interaction sites, and form transformation of chromium. Strain SL2 could sequentially remove Cr(VI) in the bioreactor. Oxalic acid produced by the fungus contributed to Cr(VI) reduction. Scanning transmissiony X-ray microscop (STXM) analysis suggested strain SL2 could partly reduce Cr(VI) to Cr(III) in the cell. Amine, carboxyl, and phosphate groups were related to Cr(VI) removal. Chromium K-edge X-ray absorption near edge structure (XANES) analysis implied Cr(III)-Cys potentially acted as an intermediate for the formation of chromium oxalate complexes during the process of treatment. This study would support the application of strain SL2 in Cr(VI) bioremediation and expand knowledge on the interaction of chromium with fungus.
利用微生物进行六价铬污染的生物修复是降低其环境风险的一种有前途的方法。本研究的目的是阐明草酸青霉 SL2 对 Cr(VI)的去除作用，包括代谢物的间接 Cr(VI)还原、相互作用位点和铬的形态转化。菌株 SL2 可以在生物反应器中顺序去除 Cr(VI)。真菌产生的草酸有助于 Cr(VI)的还原。扫描透射 X 射线显微镜（STXM）分析表明，菌株 SL2 可以在细胞内部分将 Cr(VI)还原为 Cr(III)。胺基、羧基和磷酸基团与 Cr(VI)的去除有关。铬 K 边 X 射线吸收近边结构（XANES）分析表明，在处理过程中，Cr(III)-Cys 可能作为形成草酸铬配合物的中间产物。本研究将支持菌株 SL2 在 Cr(VI)生物修复中的应用，并扩展对铬与真菌相互作用的认识。
