College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
Meat Sci. 2020 Aug;166:108112. doi: 10.1016/j.meatsci.2020.108112. Epub 2020 Mar 14.
This study was performed to reveal mitochondria changes and metabolome differences between bovine longissimus lumborum (LL) and psoas major (PM) muscles within the first 24 h postmortem. PM showed significant lower (P < .05) pH than LL at 6 h and 12 h postmortem. LL had significant higher (P < .05) mitochondrial membrane potential and lower mitochondrial reactive oxygen species levels than PM at 24 h postmortem. For metabolome, distinctive separation between LL and PM could be seen from principal component analysis and hierarchical clustering analysis. Carnosine, L-histidine and adenosine diphosphate were found with higher abundances in LL. Whereas higher abundances of inosine and hypoxanthine were detected in PM, which indicated distinct purine metabolism rate between muscle types. Higher abundances of glycerophospholipids in PM may increase risk of oxidation damage, and subsequently result in inferior meat quality during extended retail display. The results of this study provided an idea for further research regarding the muscle-specific mitochondrial ROS on meat quality.
本研究旨在揭示宰后 24 小时内牛背最长肌（LL）和腰大肌（PM）之间的线粒体变化和代谢组差异。宰后 6 小时和 12 小时时，PM 的 pH 值显著低于 LL（P<.05）。与 PM 相比，LL 在宰后 24 小时具有更高的线粒体膜电位和更低的线粒体活性氧水平（P<.05）。对于代谢组学，主成分分析和层次聚类分析显示，LL 和 PM 之间存在明显的分离。在 LL 中发现肌肽、L-组氨酸和二磷酸腺苷的丰度更高。而 PM 中检测到次黄嘌呤和肌苷的丰度更高，这表明两种肌肉类型的嘌呤代谢率不同。PM 中甘油磷脂的丰度较高，可能会增加氧化损伤的风险，进而导致在延长的零售展示过程中肉品质下降。本研究的结果为进一步研究肌肉特异性线粒体 ROS 对肉质的影响提供了思路。
