Koprivová H, Novák F, Poucková P, Dvorák P, Seblová J, Svihovcová P, Dusková J, Pospísil J, Dienstbier Z
Institute of Biophysics and Nuclear Medicine, Faculty of General Medicine, Charles University Prague, CSSR.
Int J Biol Markers. 1988 Jul-Sep;3(3):159-64. doi: 10.1177/172460088800300303.
Affinity-purified antibodies against human placental ferritin and their F(ab)2 fragments labeled with 131I were examined for suitability for radioimmunodetection of ferritin-containing tumors. The nude mouse model (BALB/c, nu/nu) with xenografts of HeLa cell tumors and human adenocarcinoma of the rectum (with proven ferritin content) was used. Gamma-camera imaging and tissue distribution studies revealed that both kinds of tumor selectively accumulate antiferritin antibodies and their fragments. In large necrotic tumors nonspecific uptake of radiolabeled normal IgG occurred, but otherwise there was no tumor localisation. This study, in accordance with the literature, confirms the utility of antiferritin antibodies for the detection of human tumors in an animal model.
对针对人胎盘铁蛋白的亲和纯化抗体及其用¹³¹I标记的F(ab)₂片段进行了检测，以评估其对含铁血蛋白肿瘤进行放射免疫检测的适用性。使用了带有HeLa细胞瘤异种移植物和人直肠腺癌（已证实含铁蛋白）的裸鼠模型（BALB/c，nu/nu）。γ相机成像和组织分布研究表明，这两种肿瘤都选择性地积累抗铁蛋白抗体及其片段。在大的坏死肿瘤中，发生了放射性标记的正常IgG的非特异性摄取，但除此之外没有肿瘤定位。本研究与文献一致，证实了抗铁蛋白抗体在动物模型中检测人类肿瘤的实用性。
