Zan Ting, Li Liqun, Xie Tingting, Zhang Li, Li Xuejun
State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
Genomics. 2020 Sep;112(5):3794-3802. doi: 10.1016/j.ygeno.2020.04.007. Epub 2020 Apr 15.
ASR (ABA-stress-ripening) genes play important roles in regulating plant growth and stress responses. This study identified 29 ASR genes in wheat. 23 pairs of tandem duplication genes and six pairs of segmental duplication genes were found in wheat ASR (TaASR) gene family, respectively. It is speculated that gene duplication event is the main driving force of TaASR genes evolution. Using published RNA-seq data and the qRT-PCR results of 12 TaASR genes, we analyzed the expression profiles for TaASR genes under abiotic stresses. It found that most of the genes mainly responded to salt and low temperature stress. Finally, subcellular localization and self-activation experiments showed that the proteins encoded by 12 TaASR genes were all located in the nucleus and cell membrane, and the full-length proteins had self-activation activity, which supported their role as transcription factors. This study provides a scientific basis for a comprehensive understanding of the TaASR gene family.
ASR（脱落酸胁迫成熟）基因在调节植物生长和胁迫反应中发挥着重要作用。本研究在小麦中鉴定出29个ASR基因。在小麦ASR（TaASR）基因家族中分别发现了23对串联重复基因和6对片段重复基因。推测基因复制事件是TaASR基因进化的主要驱动力。利用已发表的RNA测序数据和12个TaASR基因的qRT-PCR结果，我们分析了TaASR基因在非生物胁迫下的表达谱。发现大多数基因主要对盐胁迫和低温胁迫作出反应。最后，亚细胞定位和自激活实验表明，12个TaASR基因编码的蛋白质均定位于细胞核和细胞膜，且全长蛋白质具有自激活活性，这支持了它们作为转录因子的作用。本研究为全面了解TaASR基因家族提供了科学依据。
