Department of Agricultural Sciences, Nutritional Sciences, and Environmental Management, University of Giessen, Wilhelmstr. 20, 35392 Giessen, Germany.
Mitochondrion. 2020 Jul;53:21-29. doi: 10.1016/j.mito.2020.04.006. Epub 2020 Apr 15.
Melatonin blood levels vary depending on the circadian rhythm. It also stimulates antioxidant enzymes and has positive effects on mitochondrial function. The current study investigated the effects of endogenously released melatonin on adenosine triphosphate (ATP) levels and mitochondrial respiration in peripheral blood mononuclear cells (PBMC). The current study included 20 healthy adults (mean age 25,7 ± 3.4 years). Blood was collected at 8 a.m. and 2 p.m. The activity of mitochondrial respiratory complexes and ATP levels were determined in isolated PBMC. Melatonin concentrations were determined in serum samples. Sleep behavior was assessed. In PBMCs isolated from blood samples of males, respiration of mitochondrial complex IV and ATP levels as well as serum melatonin concentration were significantly lower at 2 a.m. compared to the samples collected at 8 p.m. Mitochondrial parameters and melatonin blood levels were equal at both time points in the samples isolated from females. Although our results show that the amount of melatonin secreted may have had an influence, further investigation is needed to determine the importance of melatonin and other factors in measuring the mitochondrial function of PBMC.
褪黑素的血液水平随昼夜节律而变化。它还能刺激抗氧化酶，对线粒体功能有积极影响。本研究旨在调查内源性褪黑素对周围血单个核细胞（PBMC）中三磷酸腺苷（ATP）水平和线粒体呼吸的影响。本研究纳入 20 名健康成年人（平均年龄 25.7±3.4 岁）。分别于上午 8 点和下午 2 点采集血液。在分离的 PBMC 中测定线粒体呼吸复合物的活性和 ATP 水平。在血清样本中测定褪黑素浓度。评估睡眠行为。与晚上 8 点采集的样本相比，男性血液分离的 PBMC 中，线粒体复合物 IV 的呼吸、ATP 水平以及血清褪黑素浓度在凌晨 2 点显著降低。在女性分离的样本中，两个时间点的线粒体参数和褪黑素血水平相等。尽管我们的结果表明分泌的褪黑素数量可能有影响，但需要进一步研究以确定褪黑素和其他因素在测量 PBMC 线粒体功能中的重要性。
