杏仁皮中富含酚类物质的提取物的特性、生物利用度及其对 Caco-2 细胞中促氧化剂诱导毒性的保护作用。
Characterization, bioavailability and protective effects of phenolic-rich extracts from almond hulls against pro-oxidant induced toxicity in Caco-2 cells.
机构信息
Beijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul 55108, USA.
Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul 55108, USA; Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108, MN, USA.
出版信息
Food Chem. 2020 Aug 30;322:126742. doi: 10.1016/j.foodchem.2020.126742. Epub 2020 Apr 2.
Almond hulls, the main by-product of almond production, are considered a valuable source of bioactive phenolic compounds. This study aimed to characterize the phenolic composition, bioavailability of the phenolic-rich extracts from almond hulls (PEAH), and their protective effect on oxidative stressed Caco-2 cells induced by tert-butylhydroperoxide (t-BOOH). The ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS) analysis detected 11 phenolic compounds in the PEAH with high total phenolic content and antioxidant activity. Oxidative Caco-2 cell damage was reduced by PEAH, especially at 5 μg/mL, through scavenging reactive oxygen species (ROS), modulating the cellular endogenous antioxidant system and cell redox at a predictable status. Also, in vitro digestion influenced the phenolic compounds' composition and antioxidant power of PEAH. These results suggested that almond hulls, rich in phenolic compounds, can meliorate the oxidative stressed Caco-2 cells and restore its impaired redox balance, and ultimately improve health benefits.
杏仁壳是杏仁生产的主要副产品，被认为是生物活性酚类化合物的有价值来源。本研究旨在对杏仁壳中富含酚类提取物（PEAH）的酚类成分、生物利用度及其对叔丁基过氧化物（t-BOOH）诱导的氧化应激 Caco-2 细胞的保护作用进行表征。超高效液相色谱-串联质谱（UPLC-MS）分析检测到 PEAH 中含有 11 种酚类化合物，总酚含量和抗氧化活性较高。PEAH 通过清除活性氧（ROS）、调节细胞内源性抗氧化系统和细胞氧化还原状态来减少氧化应激 Caco-2 细胞的损伤，特别是在 5μg/mL 时。此外，体外消化还影响 PEAH 中酚类化合物的组成和抗氧化能力。这些结果表明，富含酚类化合物的杏仁壳可以改善氧化应激的 Caco-2 细胞，并恢复其受损的氧化还原平衡，从而提高健康益处。
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