School of Renewable Energy, North China Electric Power University, Beijing, 102206, China.
Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
Environ Sci Pollut Res Int. 2020 Jul;27(19):24357-24368. doi: 10.1007/s11356-020-08824-y. Epub 2020 Apr 18.
The modified FeO nanoparticles were used as a support for the immobilization of horseradish peroxidase (HRP). The immobilized enzyme (HRP@FeO) was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectrometer (FTIR), and vibration sample magnetometer (VSM). According to the results, the optimum concentration of glutaraldehyde (GA) and agitation time were 300 μL and 7 h. HRP was well loaded on the surface of the FeO. There was no change in the crystal structure of HRP@FeO compared with FeO. The removals of bisphenol A (BPA) and 17α-ethinylestradiol (EE2) using HRP@FeO had been investigated. The degradation efficiencies of BPA and EE2 catalyzed by HRP@FeO were higher than that of soluble HRP. In addition, HRP@FeO can be reused through magnetic separation. After the fifth repeated use, the removal efficiencies of BPA and EE2 were up to 56% and 48%, respectively. Batch studies of catalyzed oxidation and coagulation on the degradation of BPA and EE2 in the presence of humic acid (HA) were also investigated. The order of the removal efficiencies was HRP+PACl (polyaluminum chloride)+SDS (lauryl sodium sulfate)>HRP+PACl>HRP>HRP+PAM (Polyacrylamide)>HRP+PAM+SDS. The coagulation effect of HRP@FeO and PACl was better than that of HRP@FeO and PAM. The removals of BPA and EE2 were 90.3% and 64.5% by use HRP@FeO and PACl as coagulant, while the removals were 78.7% and 57.6% by use HRP@FeO and PAM as coagulant. SDS had a positive effect on PACl, while a negative effect on PAM. Moreover, the products generated by enzymatic oxidation reaction can be effectively removed after coagulation.
改性 FeO 纳米粒子被用作辣根过氧化物酶（HRP）固定化的载体。固定化酶（HRP@FeO）通过透射电子显微镜（TEM）、X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和振动样品磁强计（VSM）进行了表征。结果表明，戊二醛（GA）的最佳浓度和搅拌时间分别为 300 μL 和 7 h。HRP 很好地负载在 FeO 的表面上。与 FeO 相比，HRP@FeO 的晶体结构没有变化。研究了 HRP@FeO 对双酚 A（BPA）和 17α-乙炔基雌二醇（EE2）的去除。HRP@FeO 催化 BPA 和 EE2 的降解效率高于可溶性 HRP。此外，HRP@FeO 可以通过磁性分离回收。重复使用五次后，BPA 和 EE2 的去除效率分别达到 56%和 48%。还研究了 HRP@FeO 存在腐殖酸（HA）时催化氧化和混凝对 BPA 和 EE2 降解的批量研究。去除效率的顺序为 HRP+PACl（聚合氯化铝）+SDS（十二烷基硫酸钠）>HRP+PACl>HRP>HRP+PAM（聚丙烯酰胺）>HRP+PAM+SDS。HRP@FeO 和 PACl 的混凝效果优于 HRP@FeO 和 PAM。使用 HRP@FeO 和 PACl 作为混凝剂时，BPA 和 EE2 的去除率分别为 90.3%和 64.5%，而使用 HRP@FeO 和 PAM 作为混凝剂时，BPA 和 EE2 的去除率分别为 78.7%和 57.6%。SDS 对 PACl 有积极影响，而对 PAM 有负面影响。此外，酶氧化反应生成的产物经混凝后能有效去除。
