Glycobiology and Bioimaging Laboratory, Research Centre for Infectious Diseases, Faculty of Medicine, Laval University and GlycoNET, Quebec, Canada.
Methods Mol Biol. 2020;2132:39-54. doi: 10.1007/978-1-0716-0430-4_5.
Galectins are a family of soluble β-galactoside-binding proteins that share conserved carbohydrate recognition domain. Galectins are found in most multicellular organisms and exert various biological functions by binding to the surface glycoconjugates as lectins. In this chapter, we describe the general methods of purification of galectins, quality control of purified galectins, some example methods of evaluating their carbohydrate-binding activity, and use of galectin to search or detect galectin ligands as well as a series of precautions for the usage of galectins.
半乳糖凝集素是一类可溶性β-半乳糖苷结合蛋白，它们具有保守的碳水化合物识别结构域。半乳糖凝集素存在于大多数多细胞生物中，通过作为凝集素与细胞表面糖缀合物结合而发挥各种生物学功能。在本章中，我们描述了半乳糖凝集素的一般纯化方法、纯化半乳糖凝集素的质量控制、评估其碳水化合物结合活性的一些示例方法、以及使用半乳糖凝集素搜索或检测半乳糖凝集素配体的方法，以及使用半乳糖凝集素时的一系列注意事项。
