糖识别与 P 型凝集素的应用。
Glycan Recognition and Application of P-Type Lectins.
机构信息
Department of Medicinal Biotechnology, Institute for Medicinal Research, Graduate School of Pharmaceutical Sciences, Tokushima University, Tokushima, Japan.
出版信息
Methods Mol Biol. 2020;2132:267-276. doi: 10.1007/978-1-0716-0430-4_26.
Cation-dependent mannose 6-phosphate receptor (CD-MPR) and cation-independent MPR (CI-MPR) belong to the P-type lectin family. Both intracellular and cell surface MPRs can recognize and bind with the terminal mannose 6-phospahte (M6P) residues of N-glycans attached to the mammalian lysosomal enzymes and the related co-factors. Domain9 (Dom9), which is one of the extracytoplasmic region of the CI-MPR, has relatively higher affinity for M6P residues. Here we describe the production of recombinant Dom9-His protein by Pichia pastris, purification, and application as a probe for lectin blotting.
阳离子依赖型甘露糖 6-磷酸受体 (CD-MPR) 和阳离子非依赖型 MPR (CI-MPR) 属于 P 型凝集素家族。细胞内和细胞表面的 MPR 都可以识别和结合附着在哺乳动物溶酶体酶和相关辅助因子上的 N-糖链末端的甘露糖 6-磷酸 (M6P) 残基。CI-MPR 的细胞外区的一个结构域 9（Dom9）对 M6P 残基具有相对较高的亲和力。本文描述了通过毕赤酵母表达系统生产重组 Dom9-His 蛋白，以及该蛋白的纯化和作为凝集印迹探针的应用。
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