可溶性甘露糖结合凝集素的制备及其四聚体。

Preparation of Soluble Malectin and Its Tetramer.

机构信息

Clinical Experimental Center, First Affiliated Hospital of Jinan University, Guangzhou, China.

Clinical Medical Research Institute, Jinan University, Guangzhou, China.

出版信息

Methods Mol Biol. 2020;2132:285-294. doi: 10.1007/978-1-0716-0430-4_28.

DOI:10.1007/978-1-0716-0430-4_28

PMID:32306336

Abstract

Malectin is a membrane-anchored endoplasmic reticulum (ER)-resident lectin, which is first identified in Xenopus laevis in 2008 and highly conserved among animals. Malectin plays an important role in the quality control of glycoprotein in the ER through recognizing the GlcManGlcNAc (G2M9) oligosaccharide chain on the newly synthesized glycoproteins. In this chapter, we will describe the preparation of recombinant soluble malectin and its tetramer, which might be developed as useful tools for detection of Glcα1-3Glc containing glycans on the cell surface.

摘要

甘露糖结合凝集素是一种膜锚定的内质网（ER）驻留凝集素，于 2008 年首次在非洲爪蟾中被鉴定，在动物中高度保守。甘露糖结合凝集素通过识别新合成糖蛋白上的 GlcManGlcNAc（G2M9）寡糖链，在 ER 中糖蛋白的质量控制中发挥重要作用。在本章中，我们将描述重组可溶性甘露糖结合凝集素及其四聚体的制备，这可能成为检测细胞表面含有 Glcα1-3Glc 的聚糖的有用工具。