Department of Northern Biosphere Agriculture, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan.
Methods Mol Biol. 2020;2132:325-338. doi: 10.1007/978-1-0716-0430-4_32.
Datura stramonium seeds contain at least three chitin-binding isolectins as homo- or heterodimers of A and B subunits. This lectin has been used for the detection and isolation of sugar chains with N-acetyllactosaminyl structures on highly branched N-glycans. In terms of future diagnostic use, the development of a recombinant lectin will be the most effective approach for producing homogeneous lectin preparations. This chapter presents details of the procedure used for lectin purification and also describes a method that can be used for producing active recombinant homodimeric BB-isolectin in Arabidopsis plants.
曼陀罗种子中至少含有三种几丁质结合同工酶，它们是 A 亚基和 B 亚基的同二聚体或异二聚体。这种凝集素被用于检测和分离高度分支的 N-糖链上具有 N-乙酰乳糖胺基结构的糖链。就未来的诊断用途而言，开发重组凝集素将是生产同质凝集素制剂最有效的方法。本章介绍了凝集素纯化过程的详细步骤，并描述了一种可用于在拟南芥植物中产生有活性的重组同源二聚体 BB-同工酶的方法。
