利用来自八放珊瑚 Sinularia lochmodes 的凝集素 SLL-2 测定共生虫黄藻形态控制的生物测定法。
A Bioassay for Determining Symbiotic Zooxanthellae Shape Control Using Lectin SLL-2 from the Octocoral Sinularia lochmodes.
机构信息
Department of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan.
AIST-Osaka University Advanced Photonics and Biosensing Open Innovation Laboratory, AIST, Photonics Center Osaka University P3, Suita, Osaka, Japan.
出版信息
Methods Mol Biol. 2020;2132:369-378. doi: 10.1007/978-1-0716-0430-4_36.
Symbiosis with zooxanthellae is essential for survival of corals. Using a bioassay, we report the H-type lectin SLL-2 purified from the octocoral Sinularia lochmodes to restrict zooxanthellae form to spherical cells. However, the factor for initiating or maintaining a symbiotic relationship between a host and zooxanthellae has not been found in many corals. This bioassay is useful for evaluating the role of a lectin as a symbiosis-related factor.
与虫黄藻的共生对于珊瑚的生存至关重要。本研究使用生物测定法，从八放珊瑚 Sinularia lochmodes 中纯化出 H 型凝集素 SLL-2，该凝集素可将虫黄藻的形态限制为球形细胞。然而，在许多珊瑚中，尚未发现启动或维持宿主与虫黄藻共生关系的因子。该生物测定法可用于评估凝集素作为共生相关因子的作用。
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