Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Methods Mol Biol. 2020;2132:641-652. doi: 10.1007/978-1-0716-0430-4_55.
Many viruses utilize cell-surface glycans as receptors for host cell entry. Viral surface glycoproteins specifically interact with glycan motifs, which strongly contributes to viral tropism. Recently, the interactions between host cell glycan receptors and the mumps virus (MuV) hemagglutinin-neuraminidase (MuV-HN) protein were characterized by determining the co-crystal structure of MuV-HN in complex with glycan receptors. Here, we describe protocols for large-scale expression, purification and crystallization of MuV-HN proteins for structural analyses and glycan-binding assays with the overarching goal of investigating glycan-protein interactions.
许多病毒利用细胞表面糖作为宿主细胞进入的受体。病毒表面糖蛋白特异性地与糖基化模式相互作用，这强烈促进了病毒的嗜性。最近，通过确定腮腺炎病毒（MuV）血凝素神经氨酸酶（MuV-HN）蛋白与糖受体的共晶结构，研究了宿主细胞糖受体与 MuV-HN 蛋白之间的相互作用。在这里，我们描述了大规模表达、纯化和结晶 MuV-HN 蛋白的方案，用于结构分析和糖结合测定，以研究糖-蛋白相互作用。
