双色蜡蘑中tectonin 2的表达、纯化及功能特性：一种对O-甲基化聚糖具有特异性的六叶β-螺旋桨凝集素

Expression, Purification, and Functional Characterization of Tectonin 2 from Laccaria bicolor: A Six-Bladed Beta-Propeller Lectin Specific for O-Methylated Glycans.

作者信息

Wohlschlager Therese, Titz Alexander, Künzler Markus, Varrot Annabelle

机构信息

Department of Biology, Institute of Microbiology, Swiss Federal Institute of Technology (ETH), Zürich, Switzerland.

Department of Biosciences, Bioanalytical Research Labs, University of Salzburg, Salzburg, Austria.

出版信息

Methods Mol Biol. 2020;2132:669-682. doi: 10.1007/978-1-0716-0430-4_58.

DOI:10.1007/978-1-0716-0430-4_58

PMID:32306366

Abstract

Tectonins are conserved defense proteins of innate immune systems featuring a β-propeller fold. Tectonin 2 from Laccaria bicolor, Lb-Tec2, is the first fungal representative of the tectonin superfamily that has been described. In-depth characterization revealed a specificity for O-methylated glycans and identified a unique sequence motif and binding site architecture underlying this unusual specificity. This chapter provides information on how to produce and purify recombinant Lb-Tec2, characterize its interaction with O-methylated glycans and demonstrate its biological function.

摘要

构建素是具有β-螺旋桨折叠结构的固有免疫系统中保守的防御蛋白。双色蜡蘑的构建素2（Lb-Tec2）是构建素超家族中首个被描述的真菌代表。深入表征揭示了其对O-甲基化聚糖的特异性，并确定了这种异常特异性背后独特的序列基序和结合位点结构。本章提供了有关如何生产和纯化重组Lb-Tec2、表征其与O-甲基化聚糖的相互作用以及证明其生物学功能的信息。