Wang Y, Gu Y Y, Fang R D, Mei Y
School of Public Health, Wuhan University of Science and Technology, Wuhan 430065, China; Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Wuhan 430064, China.
School of Public Health, Wuhan University of Science and Technology, Wuhan 430065, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2020 Mar 20;38(3):216-218. doi: 10.3760/cma.j.cn121094-20190718-00313.
To establish a method for the determination of manganese in urine with graphite furnace atomic absorption spectrometry (GFAAS) by using ionic liquid microextraction. The ethanol, 8-hydroxyquinoline and ionic liquid 1-octyl-3-methyl-imidazolium hexafluorophosphate were used as dispersive solvent, chelating agent and extraction solvent respectively, for the preconcentration of manganese. After the optimal extraction conditions were optimized by single factor rotations, evaluate the performance indicators such as methodological precision, accuracy, and detection limit. The linear range of urine manganese was 0.0-1.6 μg/L, and the correlation coefficient of standard curve line was 0.992, the detection limit was 0.03 μg/L, the recovery of sample spiked was 84.90%-96.50%, and the relative standard deviation was 0.36%-1.84%. The method has the advantages of low detection limit, high recovery rate and high sensitivity. It is suitable for the determination of manganese in urine samples from occupational exposure populations and the general population.
建立一种采用离子液体微萃取-石墨炉原子吸收光谱法（GFAAS）测定尿中锰的方法。分别以乙醇、8-羟基喹啉和离子液体1-辛基-3-甲基咪唑六氟磷酸盐作为分散溶剂、螯合剂和萃取溶剂，对锰进行预富集。通过单因素轮换法优化最佳萃取条件后，评价方法精密度、准确度和检出限等性能指标。尿锰线性范围为0.0～1.6 μg/L，标准曲线相关系数为0.992，检出限为0.03 μg/L，加标回收率为84.90%～96.50%，相对标准偏差为0.36%～1.84%。该方法具有检出限低、回收率高、灵敏度高的优点，适用于职业暴露人群和普通人群尿样中锰的测定。
