Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
BMC Complement Med Ther. 2020 Apr 19;20(1):115. doi: 10.1186/s12906-020-02906-7.
In the present study, the poncirin which is flavonoid-7-o-glycosides (isolated from the Poncirus trifoliata) in nature was evaluated against the Carbon tetra chloride (CCL4)-induced liver injury. The poncirin have been reported for various anti-inflammatory, analgesic activity etc. Based on the previous studies it was anticipated that the poncirin will ameliorate CCL4-induced liver injury.
The CCL4-induced acute and chronic liver injury model (albino BALB/c mice) was used. Following the induction of the liver injury various parameters such as food and water intake, body weight and weight to dry ratio changes were assessed. Furthermore, various hematological, biochemical parameters and histological studies such as hemotoxylin and eosin (H and E) staining were performed. The poncirin treatment was also evaluated against the pro-inflammatory cytokines such as interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) using enzyme link immunosorbant assay (ELISA). The Swiss Target prediction software was used to investigate interaction of the poncirin on the various hepatic enzymes.
The poncirin treatment markedly improved the behavioral parameters such as food and water intake. The liver weight variation was attenuated and total body was improved markedly. The hematological and biochemical parameters were significantly improved compared to the CCL4 treated groups. The anti-oxidants were induced, while oxidative stress markers were reduced promisingly. The H and E staining showed that poncirin treatment significantly improved the histology of liver compared to the CCL4 treated group. Furthermore, the poncirin treatment also evidently decreased the inflammatory mediators.
The poncirin treatment showed marked improvement in behavioral, biochemical and histological parameters following CCL4-induced liver injury. Additionally, the poncirin treatment also markedly improved the antioxidant enzymes, attenuated the oxidative stress markers and inflammatory cytokines.
在本研究中，蓬草苷（一种天然存在的类黄酮-7-O-糖苷，从枳椇中分离得到）被评估用于对抗四氯化碳（CCl4）诱导的肝损伤。蓬草苷已被报道具有各种抗炎、镇痛等活性。基于先前的研究，预计蓬草苷将改善 CCl4 诱导的肝损伤。
使用 CCl4 诱导的急性和慢性肝损伤模型（白化 BALB/c 小鼠）。在诱导肝损伤后，评估了各种参数，如食物和水的摄入、体重和体重与干重比的变化。此外，还进行了各种血液学、生化参数和组织学研究，如苏木精和伊红（H 和 E）染色。还使用酶联免疫吸附测定（ELISA）评估了蓬草苷对促炎细胞因子如白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的作用。瑞士靶向预测软件被用于研究蓬草苷与各种肝酶的相互作用。
蓬草苷治疗显著改善了行为参数，如食物和水的摄入。肝重变化得到缓解，总体重明显改善。与 CCl4 处理组相比，血液学和生化参数显著改善。抗氧化剂被诱导，而氧化应激标志物显著降低。H 和 E 染色显示，与 CCl4 处理组相比，蓬草苷治疗显著改善了肝组织学。此外，蓬草苷治疗还明显降低了炎症介质。
蓬草苷治疗在 CCl4 诱导的肝损伤后，在行为、生化和组织学参数方面显示出显著改善。此外，蓬草苷治疗还显著改善了抗氧化酶，减轻了氧化应激标志物和炎症细胞因子。
