Key Laboratory of Marine Chemistry Theory and Technology, Ocean, University of China, Qingdao 266100, China.
Key Laboratory of Marine Chemistry Theory and Technology, Ocean, University of China, Qingdao 266100, China.
Harmful Algae. 2020 Mar;93:101786. doi: 10.1016/j.hal.2020.101786. Epub 2020 Mar 13.
In this study, the extraction, identification, and antialgal activity of toxic organic substances from decaying green alga Ulva prolifera, a disaster-causing alga on green tides in the Yellow Sea, were investigated. The toxic organic substances of U. prolifera were isolated by a sequence of procedures, namely, liquid-liquid extraction (LLE), silica gel chromatography column separation (SGCC), C18 solid phase extraction (C18 SPE), and acid-alkali separation (AAS) and their antialgal activities were tested against diatom Skeletonema costatum. The results showed that the 96h-EC value in each procedure was 57.00 mg•L, 12.14 mg•L, 11.70 mg•L, and 6.31 mg•L in turn. Additionally, the initial (ethyl acetate extract in LLE) and final (Organic phase A in AAS) yield of toxic organics were 318 mg•kg and 9 mg•kg (the wet weight of U. prolifera). The final active fractions were preliminary identified as five 16- and 18-carbon fatty acids and four enols using GC-MS. The study may further evaluate the effect of bioactive substances on marine life after U. prolifera green tides bloom.
本研究调查了黄海绿潮灾害性海藻浒苔（Ulva prolifera）中有毒有机物的提取、鉴定及其抑藻活性。通过液液萃取（LLE）、硅胶柱层析分离（SGCC）、C18 固相萃取（C18 SPE）和酸碱分离（AAS）等一系列程序分离浒苔中的有毒有机物，并测试其对硅藻中肋骨条藻（Skeletonema costatum）的抑藻活性。结果表明，各步骤的 96h-EC 值依次为 57.00mg·L、12.14mg·L、11.70mg·L 和 6.31mg·L。此外，有毒有机物的初始（LLE 中的乙酸乙酯提取物）和最终（AAS 中的有机相 A）收率分别为 318mg·kg 和 9mg·kg（浒苔湿重）。采用 GC-MS 对最终活性馏分进行了初步鉴定，鉴定出 5 种 16 碳和 18 碳脂肪酸和 4 种烯醇。本研究可能会进一步评估浒苔绿潮爆发后生物活性物质对海洋生物的影响。
