Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, 100048, People's Republic of China.
Bluescape Hebei Biotech Co., Ltd, Baoding, 071000, People's Republic of China.
Biochem Biophys Res Commun. 2020 Jun 11;526(4):1100-1105. doi: 10.1016/j.bbrc.2020.04.011. Epub 2020 Apr 16.
There is a growing body of evidence that abscisic acid (ABA) and the phytochrome-interacting factor (PIF) family of transcription factors interact in light signaling, the regulation of plant growth development, and adaptation to environmental stimuli. In this study, we investigate the role that PIFs play in the regulation of ABA signaling in Arabidopsis thaliana seedlings grown under long-day conditions. We showed that PIFs positively regulate ABA signaling in post-germination seedling growth. We analyzed the DNA-binding sites for PIF3 and PIF5 by DNA-affinity purification sequencing (DAP-seq) genome-wide. The DAP-seq data showed that G-box motif is the direct binding site of PIF3 and PIF5, and a number of ABA responsive genes are potential targets of PIFs, including PYL3, PYL6, PYL12, SnRK2.2, CPK4, CPK6, ABI5, ABF3, and KIN1. Our results provide a basis for understanding the mechanism for PIFs in regulating ABA signal transduction.
越来越多的证据表明，脱落酸（ABA）和光敏色素相互作用因子（PIF）家族的转录因子在光信号转导、植物生长发育的调控以及对环境刺激的适应中相互作用。在这项研究中，我们研究了 PIFs 在拟南芥幼苗在长日条件下生长过程中对 ABA 信号转导的调节作用。我们表明，PIFs 正向调节萌发后幼苗生长过程中的 ABA 信号转导。我们通过 DNA 亲和纯化测序（DAP-seq）全基因组分析了 PIF3 和 PIF5 的 DNA 结合位点。DAP-seq 数据表明，G 框基序是 PIF3 和 PIF5 的直接结合位点，许多 ABA 响应基因是 PIFs 的潜在靶标，包括 PYL3、PYL6、PYL12、SnRK2.2、CPK4、CPK6、ABI5、ABF3 和 KIN1。我们的结果为理解 PIFs 在调节 ABA 信号转导中的机制提供了基础。
