Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic.
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
J Dairy Sci. 2020 Jun;103(6):4941-4950. doi: 10.3168/jds.2019-17367. Epub 2020 Apr 16.
Dairy cow feed contains, among other ingredients, soybeans, legumes, and clover, plants that are rich in phytoestrogens. Several publications have reported a positive influence of phytoestrogens on human health; however, several unfavorable effects have also been reported. In this work, a simple, selective, and eco-friendly method of phytoestrogen isolation based on the technique of noncovalent molecular imprinting was developed. Genistein was used as a template, and dopamine was chosen as a functional monomer. A layer of molecularly imprinted polymers was created in a microtitration well plate. The binding capability and selective properties of obtained molecularly imprinted polymers were investigated. The imprinted polymers exhibited higher binding affinity toward chosen phytoestrogen than did the nonimprinted polymers. A selectivity factor of 6.94 was calculated, confirming satisfactory selectivity of the polymeric layer. The applicability of the proposed sensing method was tested by isolation of genistein from a real sample of bovine milk and combined with micellar electrokinetic capillary chromatography with UV-visible detection.
奶牛饲料中含有大豆、豆类和三叶草等成分，这些植物富含植物雌激素。有几篇出版物报道了植物雌激素对人类健康的积极影响，但也有一些不利影响的报道。在这项工作中，开发了一种基于非共价分子印迹技术的简单、选择性和环保的植物雌激素分离方法。染料木黄酮被用作模板，多巴胺被选为功能单体。在微量滴定板中形成一层分子印迹聚合物。研究了所得分子印迹聚合物的结合能力和选择性。印迹聚合物对所选植物雌激素的结合亲和力高于非印迹聚合物。计算出的选择性因子为 6.94，证实了聚合物层具有令人满意的选择性。通过从牛奶奶样的实际样品中分离染料木黄酮并与胶束电动毛细管色谱法（带紫外-可见检测）相结合，测试了所提出的传感方法的适用性。
