Dalton S J
IBERS, Aberystwyth University, Aberystwyth, Wales, UK.
Plant Cell Tissue Organ Cult. 2020;141(2):257-273. doi: 10.1007/s11240-020-01784-8. Epub 2020 Feb 19.
Slow callus growth is a barrier to efficient genetic transformation in some gramineous species. A reformulation of Murashige and Skoog (MS) medium, with additional magnesium sulphate, potassium phosphate, copper sulphate, proline and glutamine, termed WPBS medium, has been developed which improves all aspects of in vitro culture when compared with MS based media. Embryogenic callus could be produced more rapidly from responsive genotypes of sixteen cereal, forage, model and energy grass species, whether using embryos, shoot tips or proliferated meristems as explants. Three species were not transformed due to contamination or unsuitable explant, but thirteen species were transformed using an identical -mediated transformation, selection and regeneration protocol, including and . Readily transformable species such as , and and recalcitrant species such as and were reliably transformed, while two new species and viviparous were transformed at the first attempt. It is hoped that the use of WPBS media and this general transformation protocol may help to improve the efficiency of grass and cereal transformation.
在一些禾本科物种中，愈伤组织生长缓慢是高效遗传转化的一个障碍。已开发出一种改良的Murashige和Skoog（MS）培养基，添加了硫酸镁、磷酸钾、硫酸铜、脯氨酸和谷氨酰胺，称为WPBS培养基，与基于MS的培养基相比，它在体外培养的各个方面都有改进。无论使用胚、茎尖还是增殖的分生组织作为外植体，都可以从16种谷类、饲料、模式和能源草种的响应基因型中更快地产生胚性愈伤组织。由于污染或外植体不合适，有3个物种未成功转化，但使用相同的介导转化、筛选和再生方案成功转化了13个物种，包括[此处原文缺失具体物种名称]和[此处原文缺失具体物种名称]。像[此处原文缺失具体物种名称]、[此处原文缺失具体物种名称]和[此处原文缺失具体物种名称]等易于转化的物种以及像[此处原文缺失具体物种名称]和[此处原文缺失具体物种名称]等难转化的物种都被成功可靠地转化，而两个新物种[此处原文缺失具体物种名称]和胎生[此处原文缺失具体物种名称]首次尝试就实现了转化。希望WPBS培养基的使用和这个通用的转化方案可能有助于提高草类和谷类转化的效率。
