School of Public Health, Southeast University, Nanjing 210009, China.
Department of Infection Prevention and Control, Zhongda Hospital of Southeast University, Nanjing 210009, China.
Biomed Res Int. 2020 Mar 25;2020:9358290. doi: 10.1155/2020/9358290. eCollection 2020.
Dysregulations of and were found to be related to lipid metabolism abnormality, which had been proven to be one of the mechanisms of stroke. However, limited epidemiological studies explore the associations between and and the risk of stroke among patients with hypertension in China.
We aimed to investigate the associations between genetic variants in and and the risk of stroke among patients with hypertension, as well as to explore gene-gene and gene-environment interactions.
Baseline blood samples were drawn from 211 cases with stroke and 633 matched controls. Genomic DNA was extracted by a commercially available kit. Genotyping of 5 single nucleotide polymorphisms (SNPs) in (rs2989924, rs3758269, and rs2542743) and (rs57139208, rs16939881) was performed by the polymerase chain reaction assay with TaqMan probes.
Participants with the rs2989924 GG genotype were found to be with a 1.74-fold increased risk of stroke compared to those with the AA+AG genotype, and this association remained significant after adjustment for potential confounders (odds ratio (OR): 1.74, 95% confidence interval (CI): 1.23-2.46). The SNP rs3758269 CC+TT genotype was found to be with a 33% decreased risk of stroke after multivariate adjustment (OR: 0.67, 95% CI: 0.45-0.99) compared to the rs3758269 CC genotype. The significantly increased risk of stroke was prominent among males, patients aged 60 or above, and participants who were overweight and with a harbored genetic variant in SNP rs2989924. After adjusting potential confounders, the SNP rs3758269 CT+TT genotype was found to be significantly associated with a decreased risk of stroke compared to the CC genotype among participants younger than 60 years old or overweight. No statistically significant associations were observed between genotypes of rs2542743, rs57139208, or rs16939881 with the risk of stroke. Neither interactions nor linkage disequilibrium had been observed in this study.
This study suggests that SNPs rs2989924 and rs3758269 are associated with the risk of stroke among patients with hypertension, while there were no statistically significant associations between rs2542743, rs57139208, and rs16939881 and the risk of stroke being observed.
研究发现， 和 的失调与脂质代谢异常有关，脂质代谢异常已被证明是中风的机制之一。然而，有限的流行病学研究探讨了中国高血压患者中 和 与中风风险之间的关系。
我们旨在探讨 和 基因中的遗传变异与高血压患者中风风险之间的关系，并探讨基因-基因和基因-环境相互作用。
从 211 例中风患者和 633 例匹配的对照中抽取基线血样。使用市售试剂盒提取基因组 DNA。通过聚合酶链反应测定 5 个单核苷酸多态性（SNP）（rs2989924、rs3758269 和 rs2542743）和 （rs57139208、rs16939881）在 中的基因分型，采用 TaqMan 探针的聚合酶链反应。
与 AA+AG 基因型相比，rs2989924 GG 基因型的参与者中风风险增加 1.74 倍，在调整潜在混杂因素后，这种关联仍然显著（比值比（OR）：1.74，95%置信区间（CI）：1.23-2.46）。与 rs3758269 CC 基因型相比，多变量调整后发现 rs3758269 CC+TT 基因型中风风险降低 33%（OR：0.67，95%CI：0.45-0.99）。在男性、60 岁及以上的患者、超重和携带 rs2989924 遗传变异的参与者中，中风的显著风险增加。在调整潜在混杂因素后，与 CC 基因型相比，rs3758269 CT+TT 基因型在年龄小于 60 岁或超重的参与者中与中风风险显著降低相关。rs2542743、rs57139208 或 rs16939881 基因型与中风风险之间未观察到统计学显著相关性。本研究未观察到相互作用或连锁不平衡。
本研究表明，SNP rs2989924 和 rs3758269 与高血压患者中风风险相关，而 rs2542743、rs57139208 和 rs16939881 与中风风险之间无统计学显著相关性。
