间质干细胞共培养后胰岛活力和功能的变化：系统评价和荟萃分析。
Change in Viability and Function of Pancreatic Islets after Coculture with Mesenchymal Stromal Cells: A Systemic Review and Meta-Analysis.
机构信息
Department of Hepatobiliary Surgery and Organ Transplant, First Affiliated Hospital, China Medical University, No. 155, Nanjing North Street, Shenyang, 110001 Liaoning Province, China.
Department of Stem Cells and Regenerative Medicine, Shenyang Key Laboratory for Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, Ministry of Public Health, and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, No. 77 Puhe Street, Shenbei New District, Shenyang, 110122 Liaoning Province, China.
出版信息
J Diabetes Res. 2020 Mar 24;2020:5860417. doi: 10.1155/2020/5860417. eCollection 2020.
BACKGROUND
There is no clear consensus on the effect of coculture of islets with mesenchymal stem cells (MSCs) on islet function and viability.
METHODS
We conducted a meta-analysis of relevant studies to evaluate the effect of coculture of islets with MSCs on the function and viability of islets, both and . We searched PubMed, Embase, and Web of Science databases for all relevant studies that compared the effect of coculture of islets with MSCs on the function and viability of islets (language of publication: English; reference period: January 2000-May 2019). Data pertaining to islet function and viability, concentrations of some cytokines, and experimental outcomes were extracted and compared.
RESULTS
Twenty-four articles were included in the meta-analysis. In comparison to islets cultured alone, coculture of islets with MSCs was associated with a significantly higher islet viability [weighted mean difference (WMD), -15.59; -22.34 to -8.83; < 0.00001], insulin level (WMD, -5.74; -9.29 to -2.19; = 0.002), insulin secretion index (WMD, -2.45; -3.70 to -1.21; = 0.0001), and higher concentrations of interleukin-6 (WMD, -1225.66; -2044.47 to -406.86; = 0.003) and vascular endothelial growth factor (WMD, -1.19; -2.25 to -0.14; = 0.03). Direct coculture of islets and MSCs significantly increased islet viability (WMD, -19.82; -26.56 to -13.07; < 0.00001). In the experiments, coculture of islets with MSCs induced lower fasting blood glucose level (on postoperative days 21 and 28, WMD, 102.60; 27.14 to 178.05; = 0.008 and WMD, 121.19; 49.56 to 192.82; = 0.0009) and better glucose tolerance (blood glucose at 30 minutes after intraperitoneal injection of glucose, WMD, 85.92; 5.33 to 166.51; = 0.04).
CONCLUSION
Coculture of islets with MSCs improves insulin secretory function of islets and enhances islet viability. Direct coculture of two cells significantly increased islet viability. MSC-based strategy may be beneficial for clinical islet transplantation for type 1 diabetes in the future.
背景
胰岛与间充质干细胞（MSCs）共培养对胰岛功能和活力的影响尚无明确共识。
方法
我们对相关研究进行了荟萃分析，以评估胰岛与 MSCs 共培养对胰岛功能和活力的影响。我们检索了 PubMed、Embase 和 Web of Science 数据库，以获取所有比较胰岛与 MSCs 共培养对胰岛功能和活力影响的相关研究（发表语言：英语；参考时间：2000 年 1 月至 2019 年 5 月）。提取并比较了与胰岛功能和活力、某些细胞因子浓度以及实验结果相关的数据。
结果
荟萃分析共纳入 24 篇文章。与单独培养的胰岛相比，胰岛与 MSCs 共培养后胰岛活力显著更高[加权均数差（WMD），-15.59；-22.34 至-8.83； ＜0.00001]，胰岛素水平（WMD，-5.74；-9.29 至-2.19； = 0.002），胰岛素分泌指数（WMD，-2.45；-3.70 至-1.21； = 0.0001）更高，白细胞介素-6 浓度（WMD，-1225.66；-2044.47 至-406.86； = 0.003）和血管内皮生长因子（WMD，-1.19；-2.25 至-0.14； = 0.03）更高。胰岛和 MSCs 的直接共培养显著增加了胰岛活力（WMD，-19.82；-26.56 至-13.07； ＜0.00001）。在实验中，胰岛与 MSCs 共培养后空腹血糖水平更低（术后第 21 天和第 28 天，WMD，102.60；27.14 至 178.05； = 0.008 和 WMD，121.19；49.56 至 192.82； = 0.0009），葡萄糖耐量更好（腹腔注射葡萄糖后 30 分钟时血糖，WMD，85.92；5.33 至 166.51； = 0.04）。
结论
胰岛与 MSCs 共培养可改善胰岛的胰岛素分泌功能，增强胰岛活力。两种细胞的直接共培养显著增加了胰岛活力。基于 MSC 的策略可能对未来 1 型糖尿病的临床胰岛移植有益。
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