黏粒穿梭载体与单克隆抗体相结合的基因组DNA克隆方法

Method of genomic DNA cloning by the combination of cosmid shuttle vector and monoclonal antibody.

作者信息

Tagawa M, Ito T, Kanno M, Okitsu A, Sakamoto T, Imai K, Kuwabara I, Koseki H, Matsubara H, Lau Y F

机构信息

Department of Immunology, School of Medicine, Chiba University.

出版信息

Microbiol Immunol. 1988;32(10):1073-8. doi: 10.1111/j.1348-0421.1988.tb01471.x.

DOI:10.1111/j.1348-0421.1988.tb01471.x

PMID:3231129

Abstract

We report here the strategy to isolate the DNA fragment of any species origin which encodes cell surface antigen by using cosmid library transfection and cell sortings with a monoclonal antibody. We took the mouse melanoma antigen defined by monoclonal antibody as a model system and rescued the genomic DNA by in vitro packaging, showing the feasibility of this procedure.

摘要

我们在此报告一种策略，即通过使用黏粒文库转染和单克隆抗体细胞分选来分离编码细胞表面抗原的任何物种来源的DNA片段。我们以单克隆抗体定义的小鼠黑色素瘤抗原作为模型系统，并通过体外包装拯救基因组DNA，证明了该方法的可行性。