Department of Cell, Developmental, and Regenerative Biology, Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Department of Cell Biology, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico.
Methods Mol Biol. 2020;2154:197-215. doi: 10.1007/978-1-0716-0648-3_17.
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a method designed to detect interactions between chromatin and the proteins bound to it. This method has been widely used for characterizing epigenetic landscapes in many cell types; however, a limiting factor has been the requirement of a high number of cells. Here, we describe a protocol for ChIP in epidermal cells from a newborn mouse, purified by fluorescence-activated cell sorting (FACS). This protocol has been optimized specifically for prefixed, low cell numbers, resulting in enough immunoprecipitated DNA suitable for genome-wide analysis.
染色质免疫沉淀测序（ChIP-seq）是一种用于检测染色质与结合在其上的蛋白质之间相互作用的方法。该方法已被广泛用于研究多种细胞类型中的表观遗传景观；然而，一个限制因素是需要大量的细胞。本文描述了一种从新生小鼠表皮细胞中通过荧光激活细胞分选（FACS）进行 ChIP 的方案。该方案经过专门优化，适用于预固定、细胞数量较少的情况，可获得足够用于全基因组分析的免疫沉淀 DNA。
