Assistant Professor in Pathology, Department of Neuroscience Technology, College of Applied Medical Sciences - Jubail (CAMSJ), Imam Abdulrahman Bin Faisal University, Jubail Industrial City, Al Jubail P O Box 3856, Saudi Arabia.
Department of Pathology, Malankara Orthodox Syrian Church Medical College, Kolenchery, Kerala University of Health Sciences, Kerala, India.
Indian J Pathol Microbiol. 2020 Apr-Jun;63(2):235-240. doi: 10.4103/IJPM.IJPM_526_19.
Ovarian cancers remain the most lethal of all gynecological malignancies despite major developments in their treatment.
To study the rate of expression and staining patterns of p53 in various histological types and grades of epithelial ovarian tumors (EOT).
Sixty EOTs received in a tertiary care center were studied for gross, microscopy, and p53 immunohistochemistry (IHC) expression patterns. Parameters such as age, laterality of tumor, ascites, capsule rupture, tumor size, stage at presentation, metastasis, tumor grade, and number of mitosis were correlated.
Of the sixty cases studied, 23 (38.3%) were malignant. Serous carcinomas were the largest group with 17 cases (74%) followed by mucinous with 4 cases (17%) and 2 clear cell carcinomas (9%). All benign and borderline EOT were p53 negative. 65.2% of the malignancies were p53 positive and all of them were serous malignancies. 15 out of 16 high-grade serous carcinomas were p53 positive (94%), while one case was negative (6%). 10 cases (63%) showed intense diffuse positivity of more than 60% of the nucleus, while 5 cases (31%) showed aberrant null staining <5% staining of the nucleus. All mucinous, clear cell carcinomas, and the only low-grade serous carcinoma in the study were p53 negative. P53 staining had positive correlations with variables like capsule rupture, ascites, laterality, and CA 125.
The study highlights the different rates of expression and staining patterns of p53 and the need for correct interpretation of p53 IHC for the diagnosis of various EOT.
尽管卵巢癌的治疗取得了重大进展，但它仍是所有妇科恶性肿瘤中最致命的一种。
研究不同组织学类型和分级的上皮性卵巢肿瘤（EOT）中 p53 的表达和染色模式。
在一家三级保健中心，对 60 例 EOT 进行了大体检查、显微镜检查和 p53 免疫组织化学（IHC）表达模式研究。将年龄、肿瘤侧别、腹水、囊膜破裂、肿瘤大小、分期、转移、肿瘤分级和有丝分裂数等参数与 p53 的表达和染色模式相关联。
在所研究的 60 例病例中，有 23 例（38.3%）为恶性肿瘤。浆液性癌是最大的一组，有 17 例（74%），其次是黏液性癌 4 例（17%）和 2 例透明细胞癌（9%）。所有良性和交界性 EOT 的 p53 均为阴性。65.2%的恶性肿瘤 p53 阳性，且均为浆液性癌。在 16 例高级别浆液性癌中，有 15 例（94%）p53 阳性，有 1 例（6%）p53 阴性。10 例（63%）表现为强弥漫阳性，细胞核阳性比例超过 60%，而 5 例（31%）表现为异常的阴性染色，细胞核阳性比例<5%。研究中所有的黏液性、透明细胞性癌和唯一的低级别浆液性癌均为 p53 阴性。p53 染色与囊膜破裂、腹水、肿瘤侧别和 CA125 等变量呈正相关。
该研究强调了 p53 的不同表达率和染色模式，并且需要正确解释 p53 IHC 以诊断各种 EOT。
