Malik Yatharth, Omar Balram Ji, Singh Arpana
MBBS Student, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India.
Department of Microbiology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India.
J Family Med Prim Care. 2020 Feb 28;9(2):938-942. doi: 10.4103/jfmpc.jfmpc_818_19. eCollection 2020 Feb.
A descriptive study was carried out due to absence of knowledge of relation between hygiene status of vendor and extent of contamination while simultaneously adding to the pool of similar studies, which would in turn help government formulate an appropriate policy.
A sample size of 80 fruit juices were collected from 55 vendors and cultured using CLED agar; relevant biochemical tests were run and isolates confirmed using Chromogenic Agar CPS3Id. The CFU of isolates obtained from one vendor were averaged to obtain average CFU for the vendor. The vendors were interviewed and investigator's observations were also recorded. Both were used to formulate an ingenious hygiene score scale, using which vendor hygiene score (VHS) was calculated. VHS and average CFU for the vendor were statistically analyzed and Spearman's rho was calculated along with the scatter plot.
sp. (59.05%) and non- (14.28%) are the major bacterial and fungal contaminant respectively. The median VHS is -9, which is unsuitable for consumption according to HSS. Spearman's rho was -0.736, suggestive of a statistically significant negative nonlinear correlation between VHS and extent of microbial contamination in street-vended fruit juices.
The plethora of microbial contamination of street-vended fruit juices is a lucrative dimension for research in fruit juice microbiology to improve general public health, reduce disease mortality and morbidity, reduce economic losses, and to protect the general public from the risk of bioterrorism by stringent surveillance in background of limited employment opportunities and constrained livelihoods of the vendors.
由于缺乏关于摊贩卫生状况与污染程度之间关系的了解，同时也是为了增加同类研究的数量，进而帮助政府制定适当政策，开展了一项描述性研究。
从55个摊贩处收集了80份果汁样本，使用CLED琼脂进行培养；进行了相关生化试验，并使用显色琼脂CPS3Id确认分离株。对从一个摊贩处获得的分离株的菌落形成单位（CFU）进行平均，以获得该摊贩的平均CFU。对摊贩进行了访谈，并记录了调查人员的观察结果。两者都用于制定一个巧妙的卫生评分量表，并用其计算摊贩卫生评分（VHS）。对摊贩的VHS和平均CFU进行了统计分析，并计算了斯皮尔曼等级相关系数rho以及散点图。
分别为主要的细菌和真菌污染物。VHS的中位数为-9，根据卫生评分标准，这不适于食用。斯皮尔曼等级相关系数rho为-0.736，表明VHS与街头售卖果汁中的微生物污染程度之间存在统计学上显著的负非线性相关性。
街头售卖果汁中大量的微生物污染是果汁微生物学研究的一个有利可图的方面，有助于改善公众健康、降低疾病死亡率和发病率、减少经济损失，并通过在摊贩就业机会有限和生计受限的背景下进行严格监测，保护公众免受生物恐怖主义风险。
