Medical Biology Department, Ege University School of Medicine, Bornova, 35100, Izmir, Turkey.
Otorhinolaryngology Department, Ege University School of Medicine, Izmir, Turkey.
Mol Biol Rep. 2020 May;47(5):3641-3651. doi: 10.1007/s11033-020-05458-8. Epub 2020 Apr 21.
Although there are many studies on the role of PI3K/AKT/mTOR pathway and autophagy genes in the mechanism of head and neck cancer formation and prognostic significance, there is no study investigating the role of the genes in paranasal sinus carcinomas. The aim of the study was to assess the role of the PI3K/AKT/mTOR pathway and autophagy related gene expression changes in squamous cell carcinoma of paranasal sinuses with and without neck metastasis. Eight paranasal squamous cell carcinoma patients (five without and three with neck metastasis) were included. Tissues were obtained during the surgery. Total RNA was isolated from the tissues and cDNA synthesis was performed. Expression levels of the genes were determined using qRT-PCR method. The results were evaluated using the 2 method, and fold changes of the gene expression levels in primary tumor and neck metastasis tissues were calculated according to the normal tissue. Expression levels of both PI3K/AKT/mTOR pathway and positive regulators of autophagy were significantly increased in metastasis-related two groups, especially in neck metastasis tissues. The increase in PI3K/AKT/mTOR pathway and autophagy related gene expression levels may support the metastatic character in paranasal squamous cell carcinomas. This is the first study to assess autophagy related genes in paranasal sinus cancer at transcriptome-level. Support of the transcriptome-level findings by the further protein analyses will contribute to the illumination of the rare paranasal sinus cancer molecular biology.
虽然有许多关于 PI3K/AKT/mTOR 通路和自噬基因在头颈部癌症形成和预后意义中的作用的研究，但没有研究调查这些基因在副鼻窦癌中的作用。本研究旨在评估 PI3K/AKT/mTOR 通路和自噬相关基因表达变化在伴有和不伴有颈部转移的副鼻窦鳞状细胞癌中的作用。纳入了 8 例副鼻窦鳞状细胞癌患者（5 例无颈部转移，3 例有颈部转移）。在手术中获取组织。从组织中分离总 RNA 并进行 cDNA 合成。使用 qRT-PCR 方法测定基因的表达水平。使用 2 法评估结果，并根据正常组织计算原发肿瘤和颈部转移组织中基因表达水平的倍数变化。在与转移相关的两组中，PI3K/AKT/mTOR 通路和自噬正调控因子的表达水平均显著升高，尤其是在颈部转移组织中。PI3K/AKT/mTOR 通路和自噬相关基因表达水平的增加可能支持副鼻窦鳞状细胞癌的转移特征。这是第一项在转录组水平评估副鼻窦癌中自噬相关基因的研究。进一步的蛋白质分析对转录组水平发现的支持将有助于阐明罕见的副鼻窦癌的分子生物学。
