Institute of Biomedical Engineering, Bogazici University, Uskudar, Istanbul, Turkey.
Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Bahcesehir University, Besiktas, Istanbul, Turkey.
Med Biol Eng Comput. 2020 Jul;58(7):1431-1443. doi: 10.1007/s11517-020-02166-0. Epub 2020 Apr 21.
To the best of the authors' knowledge, testing the biocompatibility of graphene coatings can be considered as the first to demonstrate human carotid endothelial cell (HCtAEC) proliferation on Au, graphene oxide-coated Au (Au/GO), and reduced graphene oxide-coated Au (Au/rGO) surfaces. We hypothesized that stent material modified with graphene (G)-based coatings could be used as electrodes for electrical impedance spectroscopy (EIS) in monitoring cell cultures, i.e., endothelialization. Alamar Blue cell viability assay and cell staining and cell counting with optical images were performed. For EIS analysis, an EIS sensor consisting of Au surface electrodes was produced by the photolithographic technique. Surface characterizations were performed by considering scanning electron microscope (SEM) and water contact angle analyses. Results showed that GO and rGO coatings did not prevent neither the electrical measurements nor the cell proliferation and that rGO had a positive effect on HCtAEC proliferation. The rate of increase of impedance change from day 1 to day 10 was nearly fivefold for all electrode surfaces. Alamar Blue assay performed to monitor cell proliferation rates between groups, and rGO has shown the highest Alamar Blue reduction value of 43.65 ± 8.79%. Graphical abstract.
据作者所知，测试石墨烯涂层的生物相容性可以被认为是首次证明人颈动脉内皮细胞（HCtAEC）在金、氧化石墨烯涂层金（Au/GO）和还原氧化石墨烯涂层金（Au/rGO）表面的增殖。我们假设用石墨烯（G）基涂层修饰的支架材料可以用作监测细胞培养物（即内皮化）的电阻抗谱（EIS）的电极。进行了 Alamar Blue 细胞活力测定和细胞染色以及光学图像的细胞计数。对于 EIS 分析，通过光刻技术生产了由 Au 表面电极组成的 EIS 传感器。通过考虑扫描电子显微镜（SEM）和水接触角分析来进行表面特性研究。结果表明，GO 和 rGO 涂层既不妨碍电测量，也不妨碍细胞增殖，并且 rGO 对 HCtAEC 的增殖有积极影响。从第 1 天到第 10 天，所有电极表面的阻抗变化率增加了近五倍。进行 Alamar Blue 测定以监测各组之间的细胞增殖率，并且 rGO 表现出最高的 Alamar Blue 还原值 43.65 ± 8.79%。图表摘要。
