软骨鱼类组织培养：鲨鱼（尖吻斜锯牙鲨）和角鲨（白斑角鲨）脑外植体的体外生长

Elasmobranch tissue culture: in vitro growth of brain explants from a shark (Rhizoprionodon) and dogfish (Squalus).

作者信息

Garner W D

机构信息

Biomedical Research Division, Mote Marine Laboratory, Sarasota, Florida 33577.

出版信息

Tissue Cell. 1988;20(5):759-61. doi: 10.1016/0040-8166(88)90020-1.

DOI:10.1016/0040-8166(88)90020-1

PMID:3232143

Abstract

Brain explants from a Caribbean sharpnose shark (Rhizoprionodon porosus) and six spiny dogfish (Squalus acanthias) were cultured at 22 degrees C in Leibovitz L-15 medium, 1000 mOsmol, with 350 mM urea, 20 mM added NaCl, and either 2% fetal bovine serum, 2% bull shark (Carcharhinus leucas) serum, or 2% spiny dogfish serum. Cell outgrowth from explants appeared after 1 month. Cells were subcultured once with 20% survival, and were viable at termination of the trials 6 months later. Cell morphology appeared normal throughout the experiment.

摘要

取自一条加勒比尖吻鲨（多孔根齿鲨）和六条白斑角鲨的脑外植体在22摄氏度下于Leibovitz L - 15培养基（1000毫渗量，含350毫摩尔尿素、20毫摩尔添加氯化钠）中培养，培养基添加2%胎牛血清、2%牛鲨（公牛真鲨）血清或2%白斑角鲨血清。外植体在1个月后出现细胞生长。细胞传代培养一次，存活率为20%，并在6个月后的试验结束时仍存活。在整个实验过程中细胞形态看起来正常。