Altawallbeh Ghaith, Castaneda Pedro, Wennecke Gitte, Karger Amy B
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, USA.
M Health Fairview, Minneapolis, MN, USA.
Pract Lab Med. 2020 Apr 5;20:e00163. doi: 10.1016/j.plabm.2020.e00163. eCollection 2020 May.
We compared hemoglobin results from manually mixed blood gas (BG) Portex syringes (Smiths Medical) and automatically mixed PICO BG syringes (Radiometer Medical) measured on the ABL90 FLEX blood gas analyzer at the Point of Care with a laboratory hematology analyzer (XN-series, Sysmex) to determine whether automatic mixing improved the accuracy and precision of hemoglobin measurement relative to the standardized method.
Ninety-nine lithium heparin tubes and EDTA tubes were collected simultaneously from selected patients at five participating institutions. The lithium heparin tubes were then split between the Portex ABG syringe and the PICO aspirator. The Portex syringe was mixed manually according to routine procedures, while the PICO syringe was mixed automatically on the ABL90 FLEX using the automatic mixing ball. The two syringes were mixed and run on the ABL90 FLEX analyzer by the same lab personnel. Total hemoglobin results obtained from the EDTA tubes on the Sysmex XN hematology analyzer were used as the reference values.
Manual mixing demonstrated a larger scatter of hemoglobin values (R = 0.515 and Mean diff.: -0.9 g/dL) while automatic mixing at the point of care yielded a significantly better correlation (R = 0.986 and Mean diff.: -0.2 g/dL) when compared to a reference hematology analyzer.
Using the PICO syringe with automatic mixing at the point of care results in a significantly better correlation of hemoglobin with the standardized method.
我们比较了在即时检验点，使用ABL90 FLEX血气分析仪对手动混合的血气（BG）Portex注射器（史密斯医疗公司）和自动混合的PICO BG注射器（雷度米特医疗公司）中的血红蛋白检测结果，并与实验室血液分析仪（XN系列，希森美康）进行对比，以确定相对于标准化方法，自动混合是否能提高血红蛋白测量的准确性和精密度。
在五家参与机构中，从选定患者处同时采集99支肝素锂管和乙二胺四乙酸（EDTA）管。然后将肝素锂管中的血液分别注入Portex ABG注射器和PICO注射器。Portex注射器按照常规程序手动混合，而PICO注射器则在ABL90 FLEX上使用自动混合球进行自动混合。两个注射器由同一名实验室人员在ABL90 FLEX分析仪上进行混合和检测。将希森美康XN血液分析仪从EDTA管中获得的总血红蛋白结果用作参考值。
与参考血液分析仪相比，手动混合显示血红蛋白值的离散度更大（R = 0.515，平均差值：-0.9 g/dL），而即时检验点的自动混合显示出显著更好的相关性（R = 0.986，平均差值：-0.2 g/dL）。
在即时检验点使用带自动混合功能的PICO注射器，可使血红蛋白与标准化方法的相关性显著提高。
